连花清瘟胶囊抗流感病毒药效物质基础及作用机理研究

项目名称： 连花清瘟胶囊抗流感病毒药效物质基础及作用机理研究

项目编号： No.81503231

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 毕丹

作者单位： 河北省中西医结合医药研究院

项目金额： 18万元

中文摘要： 连花清瘟胶囊是运用络病理论空间概念探讨外感温病及瘟疫病机传变规律，提出积极干预治疗对策研制的组方，具有广谱抗病毒作用，动物实验和临床结果均表明对流行性感冒具有良好的治疗效果，然而其抗流感病毒的药效物质基础尚不明确，本研究拟采用UPLC-MS对连花清瘟胶囊中主要化学成分进行全面系统的表征，构建连花清瘟胶囊化学成分谱和化学成分库，利用“血清药物化学理论”结合UPLC- MS技术对动物口服连花清瘟胶囊后入血成分进行定性分析，确定体内药源性成分，并对体内药源性成分进行体外抗流感病毒评价，从而确切阐明连花清瘟胶囊的药效物质基础及作用机理。本研究不仅可为连花清瘟胶囊科学化的质量标准的制定、二次研究开发提供有力的科学依据，同时宜可为推进中药现代化，中药复方药效物质以及作用机理研究提供有效的参考。

中文关键词： 连花清瘟胶囊；抗流感病毒；药效物质基础；作用机理；血清药物化学

英文摘要： Lianhua qingwen capsules is a Chinese traditional medicine researched by the guidance of collaterals disease theory, which has the function of broad-spectrum antivirus. The animal experiment and clinical research indicated that Lianhua qingwen capsules was quite effective on influenza, however the efficacious material basis hasn’t been revealed. In this project, we will ascertain the main constituents by UPLC-MS and establish the chemicalome and ingredient library. Based on serum pharmacochemistry, we will analyze the rat serum obtained after oral administration of Lianhua qingwen capsules to ascertain the Lianhua qingwen capsules-related components, the activities of the Lianhua qingwen capsules-related components in the serum will be screened using in vitro anti-influenza virus pharmacological models to elucidate the material basis for efficacy and mechanism. Through this subject, we could not only provide a base on instituting a more scientific quarlity standerd of Lianhua qingwen capsules and its secondary development, but also help on the mordenization of traditional Chinese medicine and the research of material basis for efficacy of Chinese herbal formulas.

英文关键词： Lianhua qingwen capsules;Anti-influenza virus;Therapeutic material basis;Efficacy mechanism;Serum pharmacochemistry