VEGFR2靶向超声造影定量分析评价小鼠胰腺癌的抗肿瘤治疗效果的实验研究

项目名称： VEGFR2靶向超声造影定量分析评价小鼠胰腺癌的抗肿瘤治疗效果的实验研究

项目编号： No.81201103

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 影像医学与生物医学工程

项目作者： 裴小青

作者单位： 中山大学

项目金额： 23万元

中文摘要： 我们前期的研究显示非靶向超声造影定量分析组织的血流灌注能有效评价抗血管生成药物的疗效，动物实验和临床研究也观察到超声造影定量参数能反映化疗后肿瘤血流灌注的改变。然而，该非靶向超声造影显示胰腺癌为低血供病灶，在胰腺癌的治疗和监测中作用有限。细胞毒药物和抗血管生成药物联合治疗进展期胰腺癌的研究正在进程中，目前尚未建立简单有效，可重复使用的评价方法。鉴于胰腺腺癌中血管内皮生成因子受体2（VEGFR2）高表达，本课题采用VEGFR2靶向超声造影成像技术来评价鼠胰腺癌对不同干预组（对照组、吉西他滨组、贝伐单抗组以及吉西他滨联合贝伐单抗组）的治疗反应。通过与非靶向超声造影成像的对照，研究不同治疗组靶向超声造影定量参数与肿瘤体积的改变，微血管CD34和VEGFR2的免疫荧光定量表达水平等相关性，研究靶向超声造影定量参数监测不同药物干预的有效性及优势，为胰腺癌的实验和临床研究提供有效、无创的影像学方法。

中文关键词： 弹性成像；超声；化疗；肿瘤反应；

英文摘要： Our previous studies have demonstrated that quantitative analysis of tumor blood perfusion with contrast-enhanced ultrasound (CEUS) using untargeted agent (SonoVue) could evaluate the early effect of antiangiogenic therapy, and showed the potential to monitor changes of blood kinetics in tumors during chemotherapy in animal models as well as in clinical trials. However, untargeted CEUS provided poor assessment of anticancer therapies in pancreatic adenocarcinoma due to the tumor's hypoenhancement. Specific approach is required for this purpose. The combination of cytotoxic drug with an antiangiogenic agent( a molecule-targeted drug) in pancreatic adenocarcinoma has been extensively investigated recently. However, it is still lack of a simple and reproducible imaging approach to effectively monitor the effecacy of the combined treatment. Vascular endothelial growth factor receptor 2 (VEGFR2) is overexpressed in pancreatic carcinoma, which has been used as a target in CEUS offering a noninvasive method for evaluating angiogenesis in tumors. We will apply this method to quantitatively evaluate the response of pancreatic carcinoma to different anticancer therapies (olive oil; gemcitabine; bevacizumab; combination bevacizumab with gemcitabine) in a mouse model. We will analyze quantitative parameters of VEGFR2-targ

英文关键词： elastography；ultrasonography；chemotherapy；tumor response；