TTC复合嗜神经载体系统的构建及其在神经移植应用中的研究

项目名称： TTC复合嗜神经载体系统的构建及其在神经移植应用中的研究

项目编号： No.30872664

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 武器工业

项目作者： 刘玉光

作者单位： 山东大学

项目金额： 31万元

中文摘要： 中枢神经系统疾病包括脑损伤或脑肿瘤等，是目前医学界较难解决的问题。由于神经元属于绝对静止期细胞，目前针对神经元疾病的治疗很难奏效；另外，脑恶性肿瘤如胶质瘤由于浸润生长的特性导致手术难以彻底切除，而药物或基因疗法又较难做到靶向治疗。寻找能够靶向神经元或胶质瘤细胞的载体是非常关键的。本课题组通过利用某些对神经元或胶质瘤细胞有特异结合能力的肽段，改造载基因纳米粒，使其具有靶向性，从而达到将基因输送到特定细胞内的目的。结果发现，本组开发的纳米粒具有一定的靶向运输基因的能力，并能提高转染效率。这便为中枢神经系统的基因治疗，开拓了更广阔的道路。

中文关键词： 神经元；胶质瘤；基因治疗；纳米粒；靶向

英文摘要： There are some difficult problems to be solved about the diseases of central nervous system, including severe brain injury and malignant brain tumors. Because neurons belong to absolutely stationary, non-divided cells, so many cures to keep the vulnerable neurons alive cannot work well. Also, due to the infiltrative nature of gliomas, they are difficult to be removed completely, while chemotherapy and gene therapy can kill glioma cells but cannot target them very well. So, it is vital to find some vectors which can deliver chemical agents or gene products specifically to neurons or glioma cells. We use some peptides which have tropism to specific cells to make nanoparticles loaded with plasmid can target neurons or glioma cells. Result showed our vectors have the special target ability and can enhance the transfection efficiency of neurons and glioma cells. This conclusion maybe provides novel ways to cure severe diseases of central nervous system.

英文关键词： Neuron；Glioma；Gene therapy；Nanoparticle；Target