项目名称: 解析高羊茅RA基因(FaRA)的可变剪接和抗旱性的关系
项目编号: No.31200525
项目类型: 青年科学基金项目
立项/批准年度: 2013
项目学科: 林学
项目作者: 李雪芹
作者单位: 浙江农林大学
项目金额: 23万元
中文摘要: 干旱是制约植物生长和发育的主要环境因子,也给农林业安全生产带来极大隐患。近年越来越多的研究发现干旱和光合作用关键酶Rubisco活化酶(RA)之间有着密切的联系。本课题组前期研究发现,在原有两个RA转录本的基础上,在干旱胁迫下高羊茅会形成一个新的RA转录本,可能在干旱响应机制方面具有重要的作用。因此本研究从干旱对高羊茅RA基因(FaRA)入手,研究干旱胁迫下FaRA可变剪接的变化,克隆FaRA的第三个转录本。详细研究干旱胁迫下三个转录本不同水平上的功能差异,并以转基因为手段,在功能水平上分别解析三个转录本在干旱抗性中的作用。这些研究理论上可以进一步揭示植物的干旱适应机制,实践上可以为农业生产上培育抗旱基因型植株和提高作物抗旱的技术措施提供指导,也为其它植物的耐旱性研究寻找理论依据。
中文关键词: 高羊茅;干旱胁迫;Rubisco 活化酶;可变剪接;
英文摘要: Drought is a major environmental factor which determines not only plant growth and development, but also brings great risks to the production safety of agriculture and forestry. In recent years, more and more researches find that there is a close relationship between drought and Rubisco activase (RA, a key enzyme in photosynthesis). Preliminary studies of our group find that, on the basis of the original two RA transcripts, tall fescue will form a new RA transcript under drought stress, which may play an important role in drought response mechanism. Therefore, in the present study, we will clone the its third transcript on the basis of the preliminary studies, and study the changes of alternative splicing of tall fescue RA gene (FaRA). Addtionally, this research will transfer the three transcripts of FaRA into tall fescue chromosome DNA by the plant transgenic technology and get transgenic plants with steady inheritance. Detailed study will be carried out on the basis of functional differences among the three transcripts using these transgenic plants, in order to find out their exact function under the drought stress. These studies are expected to further reveal the drought adaptation mechanisms of plants,and hopeful in providing gudience to cultivate drought-resistant genotypes of plants and improve the drough
英文关键词: Tall fescue;Drought stress;Rubisco activase;Alternative splicing;