抗菌中药方剂清除感染细菌的新机制研究

项目名称： 抗菌中药方剂清除感染细菌的新机制研究

项目编号： No.31272598

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 穆祥

作者单位： 北京农学院

项目金额： 80万元

中文摘要： 本课题以"中(兽)医药学可能主要通过保护、激活动物体维持细胞环境的结构及其功能达到防治疾病目的的一门科学"、"中药方剂治疗细菌感染性疾病的机制在于阻断细菌毒素对微血管内皮细胞的伤害"等观点为指导，旨在探寻一条既不同于抗生素样作用、又能清除机体感染细菌的新途径，期望通过深入研究致病菌和非致病菌对感染组织中嗜中性白细胞溶菌酶系统影响的差异、细菌毒素损伤微血管内皮细胞对嗜中性白细胞溶菌酶系统的影响及中药方剂对其作用、激活嗜中性白细胞溶菌酶系统的微血管内皮细胞因子，证实抗菌中药方剂清除机体感染细菌的机制是调理微血管内皮细胞激活嗜中性白细胞溶菌酶系统，并确定激活嗜中性白细胞溶菌酶系统的细胞因子，本课题的预期成果必能有助于抗菌中药成分复方的组方原则形成，有助于新型治疗细菌病药物的研发，并最终有助于从源头上确保畜产品的安全。

中文关键词： 微血管内皮细胞；中性粒细胞；抗菌机制；白头翁汤；

英文摘要： Based on the viewpoints that the approach of preventing and treating diseases of Traditional Chinese (Veterinary) Medicine is protecting the structure of maintainign cellular environment and activating its function, and that the mechanism for treating bacterial diseases of Chinese herbal medicine prescriptions (CHMPs) is to block off bacterial toxin injury to microvascular endothelial cells (MVECs), this study aims to explore a novel way for scavenging bacteria that differs from antibiotic-like effects. We would investigate the difference of neutrophil lysozymes in tissues infected pathogenic bacteria and non-pathogenic bacteria, the effect of bacterial toxin-injured MVECs on neutrophil lysozymes and the regulation of CHMPs on that, and the cytokine activating neutrophil lysozymes. Thus, it is expected to be confirmed that the mechanism scavenging bacteria of antibacterial CHMPs is activating neutrophil lysozymes by influencing MVECs, and the cytokines producted by MVECs that activates neutrophil lysozymes is expected to be determined. The study would contribute to the establishment of formulating principle for antibacterial Chinese herbal medicinal ingredients and the development of novel drugs treating bacterial diseases, and ultimately ensure the safety of animal products at the source.

英文关键词： Microvascular endothelial cells；neutrophils；antibacterial mechanism；Pulsatilla decoction；