蓝铜蛋白(BCP)调控棉纤维细胞发育的机制研究

项目名称： 蓝铜蛋白(BCP)调控棉纤维细胞发育的机制研究

项目编号： No.31471164

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 生物科学

项目作者： 李登弟

作者单位： 华中师范大学

项目金额： 86万元

中文摘要： 棉纤维细胞起源于单个种子表皮细胞，它不仅是重要的纺织原料，也是研究细胞极性生长的理想模型之一。蓝铜蛋白(BCP)是一类铜离子结合蛋白，广泛存在于植物和微生物中，这类小分子调节蛋白在棉纤维发育中的作用目前尚无报道。在前期工作中，我们鉴定了多个棉纤维发育相关的BCP蛋白，研究发现该类蛋白能够调节PIP2 类水孔蛋白的活性。在此基础上，本项目将着重研究BCP 蛋白在棉纤维发育中的功能，拟选择体外、拟南芥和棉花三种体系研究BCP对PIP2蛋白活性的调控机理及其影响棉纤维细胞发育及品质性状的信号调控途径。本研究将有助于深入了解BCP蛋白调控棉纤维细胞发育的分子机制。同时, 也可为棉纤维品质改良提供重要科学资料及重要功能基因。

中文关键词： 棉花；蓝铜蛋白；调控；蛋白质相互作用；纤维发育

英文摘要： Cotton is the most important fiber crop for the textile industry in China. Apart from their economic importance, cotton fiber also provides a unique single-celled model system for studying regulatory mechanisms of cell elongation in the absence of cell division. Blue copper-binding proteins (BCPs), which are ancient and widely distributed throughout the microorganism and plant kingdom, include a single polypeptide chain that binds a single type I copper atom. However the molecular mechanisms of this type of proteins regulating fiber cell development still remains largely unclear. In our previous study, we identified several cotton BCP genes which are involved in fiber development. And it was found that these proteins (BCPs) can interact with PIP2 proteins and regulate their activities through yeast two-hybrid analysis. In this project, we will investigate the roles of the isolated BCPs and their signaling pathway in fiber development. The mechanism of the interaction between BCP and PIP2 proteins will also be further studied to reveal how it affects the quality of fiber through in vitro, Arabidopsis and cotton systems. This study will be benefit to understanding the molecular mechamism of fiber cell differentiation and development, and also provide some new scientific data and genes (proteins) for improving cotton fiber quality.

英文关键词： Cotton (Gossypium hirsutum);Blue Copper Binding Proteins;regulation;protine-protein interaction;fiber development