雌激素受体在骨髓间充质干细胞向牙周组织细胞分化中的调控作用研究

项目名称： 雌激素受体在骨髓间充质干细胞向牙周组织细胞分化中的调控作用研究

项目编号： No.30872913

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 轻工业、手工业

项目作者： 丁寅

作者单位： 中国人民解放军第四军医大学

项目金额： 31万元

中文摘要： 牙周组织结构破坏、牙槽骨吸收是牙周病的主要病理变化，牙周治疗的关键是重建牙周组织结构。骨髓间充质干细胞（BMSCs）具有分化为牙周膜成纤维细胞的潜力，是牙周组织工程种子细胞的理想来源。雌激素受体（ER）对BMSCs体外增殖、成骨与成脂分化具有重要影响。本课题选择大鼠BMSCs体内移植引导牙周组织再生为切入点，采用细胞培养、基因转染/沉默、动物实验、生化检测（免疫组化、RT-PCR、Western－Blotting印迹分析）、Micro-CT等技术与方法，研究牙周组织微环境中，BMSCs向牙周组织细胞分化的分子调控，及ER对BMSCs向牙周组织细胞分化的调控作用及相关机理。研究结果表明：rBMSCs表达雌激素受体ERα19982;ERβ65307;雌激素通过ER影响BMSC的成骨与成脂分化；ERα20419;进BMSCs成骨分化，ERβ20419;进BMSCs成脂肪分化。雌激素促进BMSCs的成骨分化可能与PI3K/Akt信号的激活有关。牙周膜细胞具有诱导BMSCs向牙周膜细胞分化的特性。体内实验结果表明，利用BMSCs与FG复合能够较好的修复大鼠牙槽骨缺损。以上研究结果为临床应用组织再生技术修复牙周组织缺损奠定了研究基础。

中文关键词： 雌激素；雌激素受体；骨髓间充质干细胞；牙周组织再生

英文摘要： The damage of periodontal tissue structure and resorption of alveolar bone are the main pathologic changes in periodontal diseases. The key step of the periodontal treatment is to reconstruct the structure of periodontal tissue. Bone marrow mensenchymal stem cells have the differerntial potential to periodontal ligament cells.Estrogen receptor has a significant role on BMSCs' proliferation, osteogenesis. It is still unclear that the role of ER on BMSCs in the micro environment of periodontal tissue. In this project the BMSCs were used as seed cells and transplanted to repair the alveolar bone defect. The focus of our research is on the biological charactaristics during the procedure of BMSCs differentiation to periodontal tissue cells.The cell culture,gene transduction, RT-PCR, Western blot, Micro-CT were used in this research. The results indicated that ERαd ER-βwere expressed in rBMSCs.Estrogen can influence on cells osteogenic differenciation through the PI3K/Akt signal path way.The result of rat alveolar defect model indicated that the BMSCs-FC complex can induced the regeneration of rat alveolar bone and repair the alveolar defect successfully.In addition, our research also indicated that the PDLCs can induce the BMSCs change to PDLCS in co-culture of BMSCs and PDLCs.

英文关键词： estrogen;estrogen receptor;bone marrow mensnechymal stem cell;periodontal tissue regeneration