基于仿生分子识别及DNA循环放大技术的表面增强拉曼检测肿瘤细胞及单细胞分析

项目名称： 基于仿生分子识别及DNA循环放大技术的表面增强拉曼检测肿瘤细胞及单细胞分析

项目编号： No.21505082

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 数理科学和化学

项目作者： 何鹏

作者单位： 青岛科技大学

项目金额： 21万元

中文摘要： 本项目以与人类健康密切相关的重大疾病（恶性肿瘤）的早期诊断为目标，以恶性肿瘤细胞为研究对象，结合分析化学、材料科学、纳米科学、光电子学和生物医学等交叉学科的前沿研究成果，基于适体的特异性识别作用，构建高选择性、高亲和性仿生分子纳米生物探针。设计新型、高效的DNA循环放大体系，采用表面增强拉曼分析技术，将构建的仿生分子纳米探针与循环放大技术相结合，协同增强检测信号，发展肿瘤细胞的高灵敏检测新方法，构建表面增强拉曼光谱单细胞分析技术平台，以期实现癌症的早期诊断，为揭示恶性肿瘤的致病机制、靶向治疗提供理论基础和技术支持。

中文关键词： 表面增强拉曼散射；分子设计；酶循环放大；肿瘤细胞；单细胞分析

英文摘要： Early diagnosis of cancer is vital for the successful treatment of the disease. Hence, a rapid and sensitive diagnosis is essential before the cancer is spread out to the other body organs. Nowadays, surface enhanced Raman scattering (SERS) has received considerable attention owing to its high sensitivity, “fingerprinting” ability, resistance to photobleaching, minimal sample preparation, and ease of operation. In this work, a series of novel biosensing assay systems were developed for ultrasensitive SERS detection of cancer cell. The main researches include the design of biomimetic molecular recognition nanoprobe, the study of DNA circle amplification strategy based on aptamer recognition and the single-cell analysis based on SERS. The high amplification efficiency could be produced by nanoprobe enhancement and enzyme assisted multiple cycle amplification strategy. Excellent specificity and good performance in real sample assays could be achieved due to the high affinity and the specificity of the aptamer for the target cell, which is important for its application in biological or clinical analysis. The propsed methods may hold great promise for the broad applications in the field of medical research and early cancer diagnosis.

英文关键词： surface enhanced Raman scattering;molecular designing;enzyme-based circle amplification;cancer cell;single-cell analysis