基于金属纳米颗粒@共价键有机框架的C反应蛋白免疫传感器的构建

项目名称： 基于金属纳米颗粒@共价键有机框架的C反应蛋白免疫传感器的构建

项目编号： No.21465026

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 杨云慧

作者单位： 云南师范大学

项目金额： 52万元

中文摘要： 共价键有机框架（Covalent Organic Frameworks，COFs)是继金属有机框架( MOFs)之后出现的一类新型的微孔和介孔材料，它克服了MOF材料骨架容易发生坍塌的缺点，具有结构稳定多样，孔径可控等特点。目前，心血管疾病已成为人类健康的第一杀手，而冠心病占据了其中50% 以上。C 反应蛋白( CRP)是最新发现的冠心病相关炎性因子，被广泛应用于临床心血管疾病的早期诊断及鉴别。因此，急需发展灵敏、准确，快速的CRP检测方法。本项目拟在深入研究三维COFs的合成及功能化的基础上，实现以COFs为模板合成metal@COFs,并将其作为纳米生物探针标记到CRP抗体上，研制免疫传感器，利用纳米生物探针和传感界面上生物分子与样品中CRP特异性识别过程引起的界面电化学及光度的变化，实现CRP的快速、准确测定，为冠心病的诊治提供技术平台。本研究对于保护人们的生命安全具有重要的临床意义

中文关键词： 共价键有机框架；C反应蛋白；免疫传感器；金属纳米颗粒

英文摘要： Covalent Organic Frameworks(COFs) represent an exciting new type of porous and mesoporous organic materials,which are ingeniously constructed with organic building units via strong covalent bonds，appeared after metal organic framework (metal organic framework, MOF). It overcomes the defects of MOF material including skeleton easy to collapse, unstable structure because MOFs are formed by metal ions and organic ligands through coordination bond.COFs possess structure stablity and diversity, controllable pore size etc .Since the seminal work of Yaghi and co-workers in 2005, the rapid development in this research area has attracted intensive interest from researchers with diverse expertise. At present, the cardiovascular disease has become the first killer of human health. Coronary heart disease accounted for more than 50%. C-reactive protein (CRP) is newly found as a marker of inflammation associated with Coronary heart disease. The measurement of serum C-reactive protein (CRP) levels has been given particular interest for early diagnosis and differential diagnosis in clinical cardiovascular diseases. Therefore，there is urgent need for the development of sensitive, accurate, fast detection method for C-reactio protein. On the basis of research on the synthesis and functionalization of 3D COFs, this project plans to synthesis metal nanoparticles @covalent organic framework hybrid materials (COFs) using COFs as template. Furthermore，this hybrid materials will be labeled on antibody of C- reaction protein to construct immunosensor for the accurate and fast detection of C- reaction protein, utilizing the electrochemical signal or absorbance various on the interface caused by specific recognition process between nano biological probe and biological molecules on the sensor interface and c-reactive protein in the sample. This research provides reliable technology platform for the early detection, differential diagnosis and curative effect monitor of diseases such as coronary heart disease (CHD). This study has important significance in clinic for the protection of people's lives and safety.

英文关键词： covalent organic frameworks;C- reaction protein;immunosensor;metal nanoparticles