骨髓间充质干细胞复合猪脱细胞胆管支架材料治疗猪胆管缺损的实验研究

项目名称： 骨髓间充质干细胞复合猪脱细胞胆管支架材料治疗猪胆管缺损的实验研究

项目编号： No.81500482

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 陶亮

作者单位： 南京大学

项目金额： 18万元

中文摘要： 保留oddi氏括约肌功能的组织胆管支架材料治疗胆道疾病应用前景广阔，但目前仍未寻找到一种理想的支架材料。应用合适的方法获得天然胆管细胞外基质，联合合适的种子细胞，就能克服胆管修复的血供缺乏和胆汁刺激两大影响因素。本项目拟在申请人前期开展在人工胆管构建和全肝脱细胞支架制备的研究基础上，通过脱细胞技术获得保留维持胆管功能所需的纤维成分，空间结构和细胞因子，再将转染GFP的猪骨髓MSCs种植于支架表面，体外研究支架与细胞的相互作用；最后将种植猪骨髓MSCs的猪胆管脱细胞支架用于猪胆管部分缺损和节段性缺损的修复研究，观察其对胆管缺损的治疗作用和MSCs的分化和炎症调控作用。本研究结果将为组织工程胆管的构建提供一条新的思路，并为构建具有我国自主知识产权的新型的组织工程胆管奠定研究基础。

中文关键词： 骨髓间充质干细胞；脱细胞支架；胆管缺损；猪

英文摘要： Tissue engineering bile duct matrix materials aimed at preserving the function of oddi’s sphincter have broad application prospects. However, there was no advance on developing the ideal scaffold materials. It is possible to resolve the poor blood supply and inflammation by obtaining extracellular matrix associated with proper seed cells. In this research, based on the elementary work on construction of artificial bile duct and prepare of whole liver acellular matrix, we try to obtain the bile duct acellular matrix, and seed GFP modified bone marrow derived mesenchymal stem cells on this scaffold. The interaction of scaffold and stem cells were investigated in vitro. Then, the new tissue engineering bile duct was used to treat bile duct defect. The therapeutic effect, differentiation of stem cells and inflammation regulation was investigated in vivo. The ultimate purpose of this project is to provide a new, safe, reliable, efficient treatment for bile duct defect, and lay the foundation for the development of Chinese independent intellectual property rights of new tissue engineering bile duct matrix material.

英文关键词： bone marrow derived mesenchymal stem cells;acellular matrix;bile duct defect;procine