基于蛋白质检测的悬浮态SERS基底的设计及作用机理研究

项目名称： 基于蛋白质检测的悬浮态SERS基底的设计及作用机理研究

项目编号： No.51472119

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 一般工业技术

项目作者： 卑凤利

作者单位： 南京理工大学

项目金额： 82万元

中文摘要： 针对传统表面增强拉曼光谱(SERS)基底在生理环境下原位检测中存在的不足，拟利用氧化石墨烯(GO)的可悬浮性、准平面结构、可高效负载纳米粒子等特点，将GO作为负载纳米粒子的大分子双面承载平台，制备用于蛋白质检测的GO-贵金属纳米粒子SERS复合基底。探索负载条件及组装方法，实现复合基底中纳米粒子组装形态及SERS活性位点的可控调节，得到一类适合于生理环境下稳定悬浮、且保持高活性的新型SERS复合基底，并将其应用到蛋白质的免标记检测中。研究GO与纳米粒子之间的相互作用，揭示该类基底中SERS活性位点的形成机制，及其在生理环境下稳定存在的机理。研究基于该基底的多种蛋白质的SERS光谱，探索其谱学特征，揭示蛋白质分子与基底之间的作用方式，阐明其作用机理。通过本项目研究，可为基于蛋白质检测目标的SERS基底的设计提供新的思路，并有望开发出一种生理环境下原位使用的、免标记的蛋白质检测方法。

中文关键词： SERS基底；悬浮；清洁；免标记；蛋白质检测

英文摘要： To meet the requiring of in situ detection using SERS-active substrate in physiologic system,the graphene oxide(GO),which has some remarkeble features such as a well-suspended in water, rigid planar configuration,high loading for nanoparticles and ultra-large sized plateform with both faces, will be introduced into preparation of GO-nobel metal SERS composite substrate for protein detection. Through controlling the conditions, the assembly and SERS-active sites of nanoparticles on the GO plateform will be controlled, and then the well-suspended, and high active SERS substrate will be obtained and applied to lable-free detection of protein in physiologic system. The interaction beteew GO and Nanopaticals,the formatiom of SERS active sites and stabilization mechanism in physiologic system will be carried out. The SERS spectra of protein will be investigated for revelling the interaction mechanism and joint position between the protein and substrate. Through this study, the new approach for desighing new-type SERS substrate for detection of protein will be carried out and the new method for in situ and lable-free detection of protein in physiologic system will be developed.

英文关键词： SERS substrate;suspension;cleaning;lable-free;protein detection