聚烯烃表面微观结构构建及其与血小板作用机制研究

项目名称： 聚烯烃表面微观结构构建及其与血小板作用机制研究

项目编号： No.51503073

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 一般工业技术

项目作者： 叶玮

作者单位： 淮阴工学院

项目金额： 20万元

中文摘要： 与血液接触的高分子材料必须具备良好的抗凝血性能，血小板在凝血过程中承担关键作用。材料表面微观结构及其生物活性对血小板粘附行为具有重要影响。本项目以医用聚烯烃为基体材料，充分考虑材料表面生物活性及其微观拓扑结构对血小板粘附行为的影响，利用表面光引发巯烯聚合接枝亲水性单体和生物活性分子，构建具有精确设计的微观拓扑结构的生物活性抗凝血表面；通过化学结构和拓扑结构的调控，实现生物活性微图案化表面的构建；研究聚烯烃表面血小板在多重相互作用下的时间演化和空间受限行为，血小板在生物活性微观拓扑结构表面的激活和粘附过程，从而探明血小板与材料相互作用的界面生物学机制和粘附动力学。通过本项目的研究，将建立材料表面调控血小板粘附及凝血过程的方法，为制备高性能抗凝聚烯烃材料提供理论基础，并获得具有优异性能的抗凝高分子材料。

中文关键词： 聚烯烃；巯烯聚合；表面改性；血液相容性；血小板粘附

英文摘要： Biomedical polymers that contact blood must possess anticoagulant properties. Platelets play a fundamental role in thrombus formation. Surfaces micro-structure and bioactivity have more remarkably influence on platelet adhesion. In this project, we will choose biomedical polyolefin as substrates, and fully considering the influence of surface biochemical activities and topography on platelet adhesion behavior. We will construct the well-defined anticoagulant surface of polyolefin by grafting hydrophilic monomers and bioactive molecules through thiol-ene photopolymerization. Then fabricated patterned surface by regulating the chemical compositions and topology morphologies, investigated the activation and adhesion of platelets on the specific structure of polyolefin surface from the time and spatial dimensions. This project mainly focuses on the multi-interactions between platelets and polyolefin surfaces under the conditions of time evolution and space limitation to qualitatively evaluate the platelets activation and communication on polyolefin surfaces with time and quantitatively illuminate the platelet adhesion mechanisms on different bioactive surfaces. By this project, the method to manipulate the platelet adhesion and thrombus on the polyolefin-based biomaterials will be established, and high performance polymer materials with good anticoagulation will be obtained.

英文关键词： Polyolefin;Thiol-ene polymerization;Surface modification;Hemocompatibility;Platelet adhesion