莲草直胸跳甲不同地理种群热适应性的生理生殖响应机制

项目名称： 莲草直胸跳甲不同地理种群热适应性的生理生殖响应机制

项目编号： No.31272107

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 郭建英

作者单位： 中国农业科学院植物保护研究所

项目金额： 85万元

中文摘要： 温度胁迫是昆虫种群适应性进化的动力。莲草直胸跳甲是空心莲子草的专食性天敌昆虫，本项目针对该跳甲在我国夏季种群骤减而其泰国种群具有耐热性这一现象，围绕不同地理种群莲草直胸跳甲在高温胁迫下的资源利用与种群调节这一科学问题，以泰国种群和我国种群为对象，采用种群生态、形态学、生物化学、分析化学和免疫组织化学等方法， 比较两个种群在适温和高温胁迫条件下连续多代的生命表参数，探明其热适应性差异，生活史发育和形态等生态可塑性特征；定量分析其响应高温的生理生化特征和保护机制，解析热激蛋白、多元醇、糖类化合物、保护酶等的作用；比较其响应高温的生殖行为、内生殖器官发育、卵子和卵黄发生过程及神经激素含量，解析两个种群生殖潜能差异及其内分泌调控机制。研究结果对于揭示莲草直胸跳甲不同地理种群的热适应性差异及其响应高温的资源利用权衡与种群调节机制，进行种群优化和高效利用具有重要意义，并有助于丰富昆虫适应性进化理论。

中文关键词： 地理种群；耐热性；分子机制；转录组测序；

英文摘要： Heat stress is the driving force of adaptive evolution in insects. Population dynamics of Agasicles hygrophila, a biological control agent of alligator weed, differ in different geographic populations. In China, its population decreases to a very low density in the field in summer. Whereas in Thailand, its population density keeps high even in summer, indicating that the Thailand population has heat tolerance under tropic climates. In view of this phenomenon, we will use the theories and techniques of population ecology, morphology, biochemistry, analytical chemistry and immunohistochemisty, to reveal the countermeasure between defense and reproduction of the two A. hygrophila populations under high temperatures. Life-table parameters under their optimal and stress temperatures will be compared for multiple generations. Heat shock protein gene expression, contents of defensive polyols (mannitol and sorbitol) and carbohydrate (trehalose), activities of protective enzymes (superoxide dismutase, peroxidase, catalase) in each generation will be measured. Morphology of reproductive system, development of ovary and testis, and the oogenesis of the two populations will be observed, and yolk protein level at different developmental stages will be detected after the development of vitellin antibody. Contents of neurohorm

英文关键词： geographical population；heat tolerance；molecular mechanism；RNA-seq；