罗非鱼脂肪肝病的脂肪代谢分子调控机制

项目名称： 罗非鱼脂肪肝病的脂肪代谢分子调控机制

项目编号： No.31260640

项目类型： 地区科学基金项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 黄凯

作者单位： 广西大学

项目金额： 52万元

中文摘要： 本项目针对罗非鱼养殖过程中普遍存在的脂肪肝病现状，应用分子生物学的基因芯片技术的Biotin荧光标记cDNA片段，分析和检测正常和脂肪肝病变罗非鱼肝脏基因表达图谱，并对标准图谱和病变图谱进行比较分析；根据肝脏脂肪酸合成、脂肪氧化和脂肪转运途径，对罗非鱼肝脏脂肪酸合成酶（FAS）基因、脂蛋白脂酶(LPL)基因、肝酯酶（HL）基因、烯酰辅酶A水合酶短链1（ECHS1）基因、肉碱酰基转移酶（CAT）基因克隆，进行序列同源性比较，同时分析基因结构和功能，并采用实时荧光定量PCR技术研究脂肪肝病变过程中FAS、LPL、HL、ECHS1、CAT基因的表达。以期通过营养因子的改变调节相关基因的表达，进而影响罗非鱼肝脏的脂肪代谢，最终控制罗非鱼脂肪肝的发生。

中文关键词： 罗非鱼；脂肪肝；基因克隆；基因表达；转录组

英文摘要： This research pertains to the popular status quo that of fatty liver disease during the breeding process of Tilapia. It aims at adjustment of nutrition to modify the expression of genes, in order to affect the fat metabolism in Tilapia's liver and consequently prevent its fatty liver disease. In this research application of gene chip technology based on molecular biology was used for Biotin fluorescent labeling the cDNA session technology. Then test and analyze the genetic maps of healthy Tilapia fish and those disordered with fatty liver disease while draw comparisons between the healthy and disordered one. According to the composition of fatty acid in liver, channels of lipid oxidation and transmission, clone and sequence homology analysis were conducted respectively on several genes of enzymes of Tilapia-fatty acid synthase(FAS), lipoprotein lipase(LPL), lipoprotein lipase(HL), enoyl coenzyme A hydratase short chain 1(ECHS1), carnitine acyltransferase(CAT). The genetic compositions and functions were examined based on the results while adopting the real-time PCR assay for studies on the genetic expressions of FAS, LPL, HL, ECHS1, CAT during the pathological process of fatty liver disease.

英文关键词： Tilapia；fatty liver；gene clone；gene expression；transcriptome