膜微粒提高骨髓间充质干细胞治疗心肌梗死效果及其机制研究

项目名称： 膜微粒提高骨髓间充质干细胞治疗心肌梗死效果及其机制研究

项目编号： No.81500220

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 金培峰

作者单位： 温州医科大学

项目金额： 18万元

中文摘要： 冠心病导致的心肌梗死成为威胁人类健康的首要杀手，研究证明移植间充质干细胞能有效改善心肌梗死后的心功能，但是心梗部位的缺血缺氧环境导致移植的干细胞的凋亡而造成其治疗效率的下降。寻找一种高效、安全的提高间干细胞抗凋亡能力的方法已经迫在眉睫。心梗后机体释放的存在于血液循环中的膜微粒能够通过特异性受体-配体结合或细胞融合方式，直接或间接参与细胞生物活性的调节从而达到抑制靶细胞凋亡、分化等作用。在本研究中我们利用膜微粒的这一特性来处理骨髓间充质干细胞并提高其抗凋亡能力，达到提高BMSCs移植治疗心肌梗死的治疗效果，并在不同的角度对膜微粒提高干细胞抗凋亡能力的作用机制进行研究，阐明相关的分子机制和作用靶点，从而为临床干细胞移植治疗心梗提供新的研究方向，也为膜微粒生物学研究提供新的领域。

中文关键词： 心肌梗死；膜微粒；细胞移植；骨髓间充质干细胞；细胞凋亡

英文摘要： Myocardial infarction caused by coronary heart disease has become the leading threat of human health. Pervious researches have proved that bone marrow mesenchymal stem cell transplantation can effectively improve cardiac function after myocardial infarction. But the ischemia environment in the infarction area can cause the apoptosis of the transplanted stem cells and deteriorate the treatment efficiency of cell transplantation. As a result, it’s crucial for us to look for a safe and efficient method to improve the ability of anti-apoptosis for mesechymal stem cells. The substance which named microparticles release in the blood after myocardial infarction can directly or indirectly involved in the regulation of cell biological activity through specific receptors, ligand binding or cell fusion, so as to achieve the effect of inhibitory target cell apoptosis, differentiation and so on. In this study we will use microparticles pretreat mesenchymal stem cells and improve its ability to anti-apoptosis, so as to achieve the purpose of the enhancement effect of bone marrow mesenchymal stem cell therapy for myocardial infarction. In addition, we will elucidate the molecular mechanisms of the result through the various kinds of biochemical detection, consequently provide a new research direction for clinical transplantation of stem cells to treat myocardial infarction and also provide new areas of biology research for microparticles.

英文关键词： Myocardial infarction;Microparticles;Cell transplantation;bone marrow mesenchymal stem cells;apoptosis