基于PI3K/Akt信号通路探讨大黄蛰虫丸抑制糖尿病视网膜毛细血管周细胞凋亡相关机制的研究

项目名称： 基于PI3K/Akt信号通路探讨大黄蛰虫丸抑制糖尿病视网膜毛细血管周细胞凋亡相关机制的研究

项目编号： No.81473734

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 郭承伟

作者单位： 山东中医药大学

项目金额： 72万元

中文摘要： 视网膜毛细血管周细胞位于毛细血管外周, 具有维持血管通透性及稳定性等重要的生理功能。周细胞的丧失是糖尿病视网膜病变特征性病理改变。大黄蛰虫丸治疗糖尿病视网膜病变基于中医络病理论而提出，周细胞是中医络的结构基础。我们前期研究显示，大黄蛰虫丸能明显抑制实验性糖尿病大鼠视网膜毛细血管周细胞的凋亡。PI3K/Akt信号传导系统在调节细胞凋亡方面具有极为的重要作用。本课题采用光镜、电镜、图像分析、免疫组织化学、Western blot、原位PCR、RT-PCR等技术，观察大黄蛰虫丸对实验性糖尿病大鼠干预治疗下视网膜毛细血管周细胞凋亡的形态学改变，检测视网膜组织中FI3K、Akt的含量，以及该通路上游抑制因子PTED及下游MDM2、NF-κB、FKHR、BAD、caspase-9等凋亡相关因子的表达，探讨大黄大黄蛰虫丸治疗糖尿病视网膜病变的作用机理，为中医络病络治理念提供科学依据。

中文关键词： 糖尿病视网膜病变；PI3K/AKt信号转导通路；周细胞；细胞凋亡；大黄蛰虫丸

英文摘要： The pericyte of retinal capillary cells in peripheral blood capillary, have to maintain the important physiological function such as vascular permeability and stability. Pericyte's loss is diabetic retinopathy characteristic pathological change. Rhubarb eupolyphaga pill treatment of diabetic retinopathy is based on collateral theory of traditional Chinese medicine and put forward, and pericyte is the structure of the collateral foundation of traditional Chinese medicine. Our previous study showed that the rhubarb eupolyphaga pill can significantly inhibit the experimental diabetic rats retinal capillary cell apoptosis. PI3K/Akt signaling system plays an important role in regulating apoptosis. This topic using light microscope, electron microscope, image analysis, immunohistochemistry, Western blot, in situ PCR and rt-pcr technique, observation of rhubarb eupolyphaga pill on experimental diabetic rats intervention in treatment of retinal capillary cell apoptosis morphological changes, FI3K, Akt content in detection of retinal tissue, and the pathways inhibitory factor PTED upstream and downstream MDM2, nf-kappa B, FKHR, BAD, caspase -9 the expression of apoptosis related factors, explore rhubarb eupolyphaga pill in the treatment of diabetic retinopathy mechanism of complex cure collateral of traditional Chinese medicine philosophy provided the scientific basis.

英文关键词： Diabetic retinopathy;PI3K/Akt signaling pathway;Pericyte;Apoptosis;Rhubarb eupolyphaga pill