基于目标成分敲出/敲入的贵重中药麝香药效物质辨识和质量评价方法研究

项目名称： 基于目标成分敲出/敲入的贵重中药麝香药效物质辨识和质量评价方法研究

项目编号： No.81460600

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 罗云

作者单位： 江西中医药大学

项目金额： 48万元

中文摘要： 中药质量评价是实现中药现代化的关键，而药效物质辨识则是中药质量评价的基础。由于研究思路和方法等的局限，现行的中药质评方法和模式陷入了难关药效，量而不准，难评难控的窘境。为了寻求中药药效物质辨识和质评方法的突破，本项目在中医药整体观指引下,在前期初步建立了贵重中药麝香抗炎、抗菌等生物活性评价方法的基础上，借鉴遗传工程中基因敲出/导入研究策略，开展基于目标成分敲出/敲入的麝香药效物质辨识和质评方法研究。敲出即在谱效关系辅以成分吸收研究基础上，选择性分离目标成分，再通过抗炎、抗菌、调节神经系统功能、兴奋子宫、抗凝血等生物活性检测方法逐步确认关键药效组分；敲入即在阴性样品中加入不同比例的目标成分，建立目标成分的量效关系，制订科学合理的药效组分限量范围，最终实现药效物质找得准，测得准，定得准的麝香质评目标，亦为贵重中药质量评价和珍稀濒危中药人工代用品研发提供研究思路和技术支持。

中文关键词： 麝香；药效物质辨识；质量评价；成分敲出/敲入；量效关系

英文摘要： Quality evaluation has been one of the most important and difficult issues for the modernization of traditional Chinese medicine (TCM), while efficient constituents screening supplies the prerequisite and foundation for quality evaluation. The current quality evaluation pattern for TCM could not assure the safety and efficacy of TCM because this pattern mainly focused on the qualitative and quantitative determination of chemical components which might not be exclusive or active ingredients, while the content limits of index components were set according to the content distribution of several batches of randomly chosen TCM samples. To find a breakthrough of the efficient constituents screening and quality evaluation for TCM, a new pattern based on target component knockout/knock-in strategy imitating gene knockout/transfer was introduced to accurately screen for the key efficent constituents (main efficient components and effect-related components) and explore the interaction properties of them by component knock-out strategy. Further, to set reasonable content limits of these key efficient constituents according to their quantity-efficiacy relationships by component knock-in strategy. All the results will provide some reference for meeting with the requirements of the effect-related, quantitative,accurate and assessable quality evaluation standards. In this research, moschus, an important,expensive, effective and commonly used drug,was chosen as the model TCM. This strategy of target component knockout/knock-in was consistent with the holistic characteristic of TCM, could not only identify the effective components of moschus but also evaluate the quality of it effectively, accurately. It will provide some idea and technical support for the evaluation of other important and expensive TCMs and the exploitation of their artificial substitutes.

英文关键词： Moschus;recognition of efficient constituents;quality evaluation;component knock-out/knock-in;dose-efficacy relationship