西藏冬虫夏草遗传多样性及与虫草品质相关性研究

项目名称： 西藏冬虫夏草遗传多样性及与虫草品质相关性研究

项目编号： No.31270068

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 旺姆

作者单位： 西藏大学农牧学院

项目金额： 78万元

中文摘要： 冬虫夏草是我国珍稀名贵中药材，西藏自治区虫草产区农牧民收入的80%以上来自于冬虫夏草。西藏冬虫夏草因其"地源"和"种源"的不同，品质差异较大，但目前其品质仍以外部形态特征来评价，缺少客观、科学和便捷的评价方法。本项目综合运用形态学、植物化学、分子生物学等手段，开展西藏冬虫夏草遗传多样性与冬虫夏草品质相关性研究，分析冬虫夏草菌及寄主蝙蝠蛾的遗传变异、产地环境等对西藏冬虫夏草有效活性成分的影响，阐明西藏不同"种源" 和"地源"冬虫夏草的品质特点及影响因素，筛选与冬虫夏草品质相关的特征性指标，构建科学的冬虫夏草质量评价体系，为科学、准确地鉴定冬虫夏草的等级、真伪及其品质提供理论支持和技术平台，对西藏地区冬虫夏草资源的利用与深度开发具有重要意义。

中文关键词： 冬虫夏草；遗传多样性；协同进化；核苷类成分；

英文摘要： Ophiocordyceps sinensis is one of rare and precious Chinese herbal medicines. In the O. sinensis producing areas of the Tibet Autonomous Region, more than 80% of farmers incomes were from the O. sinensis. Quality of O. sinensis which was produced in Tibet, is dissimilarity due to the differences of "provenance" and "land races". But until now, the quality evaluation of O. sinensis is still based on the external morphological characteristics. There is no a specific, scientific and convenient evaluation method using for quality evaluation. The objective of this project is that carry out study of the correlational between quality and genetic diversity of O. sinensis, as well as between its host insects, using the means of morphology, phytochemistry, molecular biology. The study will be on the effecting of environment, genetic variation of O. sinensis and host insect on activity compositions of O.sinensis, also clarify the quality characteristics and influencing factors of O.sinensis which produced in Tibetan. It is building a technology platform and providing theoretical support to make a scientific system for evaluating quality of O.sinensis, which is very important to deeper study and economic development of Tibetan O.sinensis.

英文关键词： Ophiocordyceps Sinensis；genetic diversity；coevolution；nucleosides；