基于石墨烯涂层的电喷雾解吸电离质谱技术研究及其在复杂样品分析中的应用

项目名称： 基于石墨烯涂层的电喷雾解吸电离质谱技术研究及其在复杂样品分析中的应用

项目编号： No.21305029

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 卢明华

作者单位： 河南大学

项目金额： 27万元

中文摘要： 发展快速、灵敏、实时、在线、原位与高通量的分析技术是当今分析化学发展的重要趋势。质谱技术是众多分析方法中同时具备高灵敏度和高选择性，且能够在一次分析中同时提供多个被分析物的分子量和结构信息，是分析鉴定复杂样品中未知组分不可或缺的工具。样品纯度是影响质谱信号强度的最重要因素之一，因此传统质谱分析技术往往需要对被分析样品进行净化处理和色谱分离。然而繁琐耗时的样品预处理和色谱分离大大限制了质谱技术在大量、多批次样品分析中的应用。无需样品预处理条件下直接对复杂基体样品中待测物离子化技术的出现，如电喷雾解吸电离和电喷雾萃取电离，使得复杂样品的快速质谱分析成为可能。石墨烯作为一种新型的二维碳纳米材料，近年来迅速成为物理、化学、材料、传感、生物医药等领域的研究热点。本项目拟借助石墨烯大的比表面积和良好的导电导热性能，发展一种基于石墨烯材料的直接电喷雾解吸电离离子化技术，用于复杂样品基体的直接质谱分析。

中文关键词： 石墨烯；氧化石墨烯；植物激素；食品添加剂；分散固相萃取

英文摘要： There is a great need for developing new techniques to enable fast, sensitivity, real-time, online, in situ, high throughput and accurate characterization of compounds in complex samples. Mass spectrometry (MS) is a powerful analytical technique that can provide not only high sensitivity, high selectivity and specificity, but also molecular weight and structure information. MS has been considered as an indispensable tool in the trace analysis of chemicals in complex samples. In MS analysis, the matrix of sample has a significant influence on signal intensity of the analytes. Therefore, sample pretreatment (e.g., SPE and SPME) and chromatographic separation (GC, HPLC and CE) usually were required to couple with MS in the analysis of complex samples, such as environmental and biological samples. Tedious sample preparation and chromatographic separation not only consumed a large amount of organic solvent, but also costed a lot time, which has a significant limitation of MS technique on the analysis of large amount and multi-batch samples. In 2004, Cooks's groups developed desorption electrospray ionization mass spectrometry (DESI-MS) technique that allowed the direct analysis of various samples in the open atmosphere with little or no sample preparation, which open new era for the direct analysis of complex samples

英文关键词： graphene；graphene oxide；plant hormones；food additives；dispersive solid-phase extraction