新型稀土配合物时间分辨荧光-磁共振双模式生物成像探针的合成与应用

项目名称： 新型稀土配合物时间分辨荧光-磁共振双模式生物成像探针的合成与应用

项目编号： No.21475016

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 高分子科学

项目作者： 宋波

作者单位： 大连理工大学

项目金额： 80万元

中文摘要： 可视化的生物成像技术在医学与生命科学研究及临床诊断中居于十分重要的地位，而开发和应用可用于两种或多种生物成像检测技术的多模式成像探针已成为当前分子影像学领域新的研究热点。本项目拟研制基于稀土配合物的时间分辨荧光-磁共振双模式成像探针，新探针结合了磁共振成像高组织穿透性、高空间分辨率和时间分辨荧光成像高灵敏度、高选择性的优点。在探针研制的基础上，本项目拟建立基于双模式成像的体内癌细胞靶向标识和成像检测技术，为肿瘤诊断和手术中荧光引导肿瘤切除提供理论依据和新方法。同时建立在细胞和生物活体水平上的生理活性组分的时间分辨荧光-磁共振双模式成像测定技术，为研究这些生理活性组分在体内对各种生物功能的调节作用及其与疾病的关系提供有用的工具。本研究不仅对稀土配合物生物成像探针研究的发展、开拓其在生化分析中新的应用领域具有很好的理论与实际意义，预计其研究成果在生物医学与生命科学领域也具有很好的应用前景。

中文关键词： 稀土配合物；时间分辨荧光成像；磁共振成像；生化分析；生物传感

英文摘要： Bioimaging technology that enables visible of biological molecules is a most important tool to the researches of medicine and life science, as well as clinical diagnosis. In this area the dual or multiple modality molecular imaging has exciting potentials and attracted a lot of research interests. In this project some novel multimodal TRLI (Time Resolved Luminescence Imaging)-MRI probes based on lanthanide complexes will be developed for molecular imaging in biological systems. The multimodal TRLI-MRI imaging combines the high spatial resolution and deep tissue penetration of MRI with the sensitivity and selectivity of the TRLI and integrates the advantages of both techniques. The newly designed probes can be used for multimodal TRLI-MRI imaging of cancerous cells and their markers in vitro and in vivo, which permits the preoperative visualization of tumors by serving as an MRI contrast agent and affords an intraoperative discrimination of tumors from normal tissue because of its luminescence. Furthermore, the probes also can be used for the specific recognition and multimodal TRLI-MRI imaging of bio-active molecules and ions in vitro and in vivo, which affords powerful tools for investigating their biological functions and relationships with some diseases. This project not only has important theoretical and practical significance for the development of bioimaging probes based on lanthanide complexes and deploitation of their application fields, its results have also good application prospects in biomedical and life science researches.

英文关键词： lanthanide complex;time-resolved luminescence imaging;magnetic resonance imaging;bioassay;biosensing