参与根尖干细胞维持的生长素相关关键基因RSD1的功能鉴定

项目名称： 参与根尖干细胞维持的生长素相关关键基因RSD1的功能鉴定

项目编号： No.31270327

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 丁兆军

作者单位： 山东大学

项目金额： 90万元

中文摘要： 植物高度适应周围环境的能力主要取决于以根干细胞为基础的可塑性调节。生长素和转录因子如PLTs、SCR、SHR、WOX5等已被发现是根干细胞活性调节的关键调控元件，但我们仍不清楚根干细胞维持和分化的调控网络。为了发掘更多该信号途径的调控因子，我们做了EMS诱导的突变体筛选，发现rsd1(root stem cell defective 1)能够促进根干细胞的分化和QC细胞的分裂。rsd1突变体根中DR5GUS信号的大大减弱而叶片中DR5GUS的信号大大增强。在图位克隆RSD1的基础上,我们将通过遗传分析，酵母双杂交，转录组分析等手段深入研究该基因的功能。同时，我们还将进行rsd1突变体生长素极性运输的测定及自由态生长素IAA的检测，以阐明导致rsd1突变体根和叶差异DR5GUS信号的机制。在系统研究RSD1功能的基础上，我们将为未来建立根干细胞维持和分化的调控网络大大丰富调控因子资源。

中文关键词： 根尖干细胞；静止中心；延伸因子ELP2；表观调控；

英文摘要： The highly adapting abilities of plants to the changing environmental conditions mainly depend on the plasticity of root growth and development, which is largely attributed to the root stem cell maintenance and differentiation. There are many genes including AP2 transcription factor PLETHORAs (PLTs) , GRAS transcription factors SCARECROW (SCR) and SHORTROOT (SHR), and quiescent center ( QC）specific expressed WOX5 have been found to be involved in the root stem cell maintenance and differentiation. Auxin was also found to be crucial to maintain the root stem cell identity. However, we still don't have the clear regulation network about the root stem cell maintenance and differentiation. In order to identify more regulators in this process, we performed an EMS-induced mutagenesis screening, rsd1(root stem cell defective 1) was isolated and showed promoted root stem cell differentiation and QC division which is rarely happened in wild type. The DR5GUS staining signals were strongly reduced in rsd1 roots while they were increased in rsd1 leaves. In this project, we will further investigate the functional mechanism of RSD1 after mapping this gene. To the end, the genetic analysis, yeast screening, and transcriptome analysis will be carried out. Furthermore, auxin transport assays and free IAA measurement will be perf

英文关键词： root stem cell；quiescent centre (QC)；ELP2；epigenetic regulation；