鳅超科鱼类体色体斑的演化及相关基因的进化分析

项目名称： 鳅超科鱼类体色体斑的演化及相关基因的进化分析

项目编号： No.31272306

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 唐琼英

作者单位： 中国科学院水生生物研究所

项目金额： 79万元

中文摘要： 许多鱼类具有鲜艳而多样的体色体斑，这可能与环境适应及性选择相关，也可能受相关基因的影响。鳅超科是一群美丽的中小型底栖鱼类，其体色体斑使该类群具有丰富的多样性。本项目拟采用系统发育比较方法，研究鳅超科鱼类体色体斑的演化过程，检测该特征在各类群中受系统发育限制的程度及演化速率等。测量各环境参数，采用典范对应分析探讨体色体斑与环境因子的关系。设计性选择实验，验证性选择促使近缘种或同种个体间体色体斑发生分化的假说。对体色体斑直接相关的基因和间接相关的视蛋白基因进行克隆测序，分析这些基因在鳅超科各类群中受到的选择压力及进化速率差异、在体色体斑形成中的作用、两类基因是否存在协同进化关系、基因间的分化时间等，从而研究体色体斑演化的分子基础。本项目的研究结果将有助于揭示鱼类体色体斑多样性形成的普遍规律及机制，丰富进化生物学的基础资料，也可以为观赏鱼优良品种的选育提供参考，具有重要的理论价值和实践意义。

中文关键词： 体色斑纹；环境适应；典范对应分析；系统发育比较方法；选择压力

英文摘要： The remarkable diversity seen in the color pattern of many fishes is probably derived from their adaptation to environment or sexual selection or regulation by the related genes. The superfamily Cobitoidea is a group of small to medium-size benthonic fishes with beautiful and diverse color pattern which lead to the abundant biodiversity of the Cobitoidea. In this project, the pigment pattern evolution of the Cobitoidea will be investigated using Phylogenetic Comparative Methods (PCMs) with which the pigment pattern can be estimated the phylogenetically constrained extent and evolution rate. Many environmental factors will be measured, and the association between color pattern and environmental factors will be analyzed by Canonical Correspondence Analysis (CCA). Indoor sexual selection experiments of female preference for male color will be conducted to test the hypothesis that sexual selection drives the diversification of color pattern among closely relative species or conspecific individuals.To learn about the genetic base of pigment pattern evolution, the related genes including pigment genes and opsin genes will be cloned and sequenced. Based on the obtained phylogenetic relationships of the Cobitoidea, select pressure along each Cobitoidea lineage will be analyzed for these genes with analyzing their functi

英文关键词： Color patterns；Environment adaptation；Canonical Correspondence Analysis；Phylogenetic Comparative Methods；Selection pressure