基于多糖等成分转化-性味-药效相关联的关黄柏盐炙机理研究

项目名称： 基于多糖等成分转化-性味-药效相关联的关黄柏盐炙机理研究

项目编号： No.81473351

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 王秋红

作者单位： 黑龙江中医药大学

项目金额： 73万元

中文摘要： 关黄柏味苦，性寒，功能清热燥湿、泻火解毒，盐炙可以缓和其苦燥之性，增强入肾经、滋肾阴及清肾火作用。一直以来，人们关注盐炙后关黄柏中生物碱、挥发油和黄酮成分的变化,而我们在前期的研究中，发现关黄柏富含多糖成分，盐炙后多糖的种类发生较大变化。在建立的以HPLC、UPLC-MS法分析均一多糖、以质谱、NMR等研究多糖的一级结构及以原子力显微镜观察多糖二维结构的研究模式下，发现盐炙后黄柏多糖由4种变为7种，糖链变短，且盐炙后的多糖具有显著的免疫抑制作用；依据中医临床经验总结，肾经虚热多为免疫反应发热，所以多糖极可能通过免疫抑制机制发挥入肾、清肾火作用。关黄柏中生物碱等小分子在使用各种炮制方法处理后含量均下降，如果作为唯一的药效物质，根本无法解释其盐炙炮制的意图。本研究对盐炙与多糖、生物碱等组分转化及缓和药性、清肾火相关性开展研究，以揭示盐炙关黄柏的炮制原理，并期待发现安全低毒的多糖类免疫抑制剂。

中文关键词： 关黄柏；多糖；药效物质基础；免疫抑制；炮制机理

英文摘要： Hellodendron amurense, bitter in flavor and cold in nature, possesses the properties of clearing heat with eliminating dampness and purging fire for removing toxin. The bitter and dryness of Hellodendron amurense can be reduce by salt processing which enhance the effect of reach kidney meridian, nourishing kidney yin, and clearing kidney fires at the same time. Researchers have been focusing on the chemical variances of alkaloids, volatile oil, and flavonoids from Hellodendron amurense. In contrast, however, the polysaccharides of Hellodendron amurense was found to change greatly after salt processing according to our previous researches, i.e. species of polysaccharides were reduced from 7 to 4 and their molecule weights lowered after salt processing by using the founded study model, including analysis methods of HPLC and UPLC-MS and structure identified methods of MS and NMR. Significant immunosuppressive activities of salt processed Hellodendron amurense were also found in previous researches. Asthenia heat of kidney meridian was mostly resulted from immunological reaction according to the experiences of traditional Chinese medical clinical. Thus, Hellodendron amurense polysaccharides probably performed its actions of entering kidney meridian and clearing kidney fire by immunosuppression. Too many processed methods resulted in the reduction of levels of small molecules like alkaloids in Hellodendron amurense, which is impossible to elucidated the mechanism of salt processed Hellodendron amurense as the exclusive therapeutic basis. Thus, we will study the relatives between salt processing, components of Hellodendron amurense, and pharmaceutical effects, which contributes to reveal the mechanism of salt processed Hellodendron amurense and discover safety and lower toxic polysaccharides immunosuppressive agents.

英文关键词： Hellodendron amurense;polysaccharides;therapeutic basis;immunosuppressive;mechanism of processing