妊娠期轻度碘营养不足及早期补碘对子代大鼠小脑运动性学习记忆的影响及机制研究

项目名称： 妊娠期轻度碘营养不足及早期补碘对子代大鼠小脑运动性学习记忆的影响及机制研究

项目编号： No.81502805

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 王园

作者单位： 中国医科大学

项目金额： 18万元

中文摘要： 轻度碘营养不足在妊娠期和哺乳期妇女人群中普遍存在，但其对小脑运动性学习记忆损伤的研究未见报道，机制尚不清楚。并且妊娠早期补碘能否恢复轻度碘营养不足对小脑运动性学习记忆的影响还存在争议。前期研究已成功获得轻度碘营养不足动物模型，发现妊娠期和哺乳期轻度碘营养不足可抑制小脑浦肯野细胞树突生长发育，损害颗粒细胞增殖和分化，造成小脑功能发育受损。在此基础上，我们通过控制大鼠每日低碘饲料和碘饮水的摄入量来建立妊娠期补碘动物模型，进一步研究轻度碘营养不足以及补碘后对仔鼠小脑突触可塑性和运动性学习记忆形成的影响，深入分析调控浦肯野细胞LTD及其AMPA受体相关因子的变化对仔鼠小脑运动功能的调节作用，具体阐明轻度碘营养不足以及补碘后影响小脑突触可塑性和运动性学习记忆形成的机制。为我国制定适宜补碘相关政策，对预防妊娠期和哺乳期妇女碘营养不足，提供科学依据。

中文关键词： 轻度碘营养不足；补碘；小脑；突触可塑性；运动性学习记忆

英文摘要： Mild iodine deficiency during pregnancy and lactation is widespread. However, the effects of mild iodine deficiency on cerebellar motor learning are not reported, and the mechanisms remain unclear. It is controversial that iodine supplement in the first trimester of pregnancy can recover the damage of cerebellar motor learning induced by mild iodine deficiency. Previous studies have successfully obtained the animal model of mild iodine deficiency, found that mild iodine deficiency during pregnancy and lactation can inhibit cerebellar purkinje cells dendritic growth, reduce granule cells proliferation and differentiation, and damage cerebellum functional development. On this basis, we build rat model of iodine supplement during pregnancy by iodine deficiency diet and drank iodine deionized water to further study the effects of mild iodine deficiency and iodine supplement on synaptic plasticity and motor learning in offspring cerebella. The regulating effect of cerebellar purkinje cells LTD and AMPA associated factors will be analysed. The underlying mechanisms of cerebellar synaptic plasticity and motor learning in offspring by mild iodine deficiency and iodine supplement are clarified. Results of this study may contribute to formulate appropriate iodine related policies and prevent iodine nutritional deficiency during pregnancy and lactation women in our country.

英文关键词： mild iodine deficiency;iodine supplement;cerebellum;synaptic plasticity;motor learning