从肠菌-黏膜免疫调节研究艾灸重建PI-IBS肠道稳态的效应机制

项目名称： 从肠菌-黏膜免疫调节研究艾灸重建PI-IBS肠道稳态的效应机制

项目编号： No.81503656

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 包春辉

作者单位： 上海中医药大学

项目金额： 18万元

中文摘要： 肠道菌群与黏膜免疫的失控所引发的肠道稳态的破坏已被证实是感染后肠易激综合征（PI-IBS）发生发展的关键环节。本项目在课题组前期确证针灸有效性的基础上，围绕“艾灸对PI-IBS肠道菌群多样性及NLRP6炎性体介导的肠黏膜免疫的调节是其重建肠道稳态有效治疗PI-IBS的关键效应机制”的工作假说，运用基于宏基因组学、生物信息学、组织学以及分子生物学的各项技术从临床和实验两个层面观察和验证艾灸对PI-IBS肠道菌群的调节作用，并进一步深入观察艾灸核心调控菌种与NLRP6炎性体介导的肠黏膜免疫的交互作用以及艾灸的调控机制，以期证实：①艾灸治疗PI-IBS患者临床效应的产生与其调节肠道菌群密切相关；②艾灸对PI-IBS肠道菌群及NLRP6炎性体介导的肠黏膜免疫的调节是其重建PI-IBS肠道稳态的关键效应机制，从而为临床艾灸有效治疗PI-IBS提供一定的科学依据。

中文关键词： 肠道稳态；肠道菌群；宏基因组学；艾灸疗法；NLRP6炎性体

英文摘要： The damage of intestinal homeostasis caused by the imbalance between intestinal microbiota and intestinal mucosal immunity has been proven to be the key process of post-infectious irritable bowel syndrome (PI-IBS). Base on our previous study on the effectiveness of acupuncture and moxibustion treatment, and on the hypothesis regulation of PI-IBS intestinal microbiota diversity and NLRP6 inflammasome-mediated intestinal mucosal immunity through moxibustion therapy is the key response mechanism on reconstructing intestinal homeostasis in treating PI-IBS, metagenomics, bioinformatics, histological and molecular biology techniques are applied to observe and verify the role of moxibustion in the regulation of PI-IBS intestinal microbiota, and further gain insights into interactions between the key species and NLRP6 inflammasome-mediated intestinal mucosal immunity in PI-IBS in order to confirm: ①moxibustion can exert clinical effects upon PI-IBS patients is closely related to the regulation of intestinal microbiota; ②The regulation of intestinal microbiota and NLRP6 inflammasome-mediated intestinal mucosal immunity through moxibustion on PI-IBS is the key response mechanism in the reconstruction of intestinal homeostasis, providing the scientific basis for moxibustion treating PI-IBS in the clinical practice.

英文关键词： gut homeostasis;intestinal microbiota;metagenomics;moxibustion therapy;NLRP6 inflammasome