基于DNase高通测序信息的DNA蛋白结合位点分析

项目名称： 基于DNase高通测序信息的DNA蛋白结合位点分析

项目编号： No.61471139

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 无线电电子学、电信技术

项目作者： 冯伟兴

作者单位： 哈尔滨工程大学

项目金额： 85万元

中文摘要： 作为DNA碱基代码功能分析的核心，DNA蛋白结合位点分析具有显著的重要性和迫切性。DNase高通测序可一次性在全基因组范围内检测所有种类DNA蛋白结合位点，其分辨率可达单碱基，显著优于现有检测技术。但现有研究尚仅限于利用DNase数据在DNA上对是否是DNA蛋白结合位点进行识别，无法实现上述目的。本课题创新性地提出利用该数据进一步对DNA蛋白结合位点的类别进行识别，即识别结合位点所对应结合蛋白的类别，从而获取完整的DNA蛋白结合位点信息，进而实现一次性在全基因组范围内检测所有种类DNA蛋白结合位点的目的。基于此，本课题研究新分析方法，通过数据预处理、DNA蛋白结合位点完整信息获取、DNA蛋白结合位点分离等研究，在增强有用信息基础上，分析全基因组范围内不同种类DNA蛋白结合位点的结合状态，从而为动态实时高分辨率地分析DNA上各种功能蛋白复杂的调控模式，以及更高层次生物调控机理研究创造条件。

中文关键词： 核酸分子信息；基因测序；核酸检测；蛋白结合位点

英文摘要： As core of functional analysis for DNA nucleotides, the importance and imperativeness of analysis of DNA protein binding sites is prominent. As the latest technology in detection of DNA protein binding sites, DNase high throughput sequencing (DNase-Seq) can detect binding sites for all DNA proteins in whole genome at one time, and the resolution can reach one-nucleotide level, which makes it the most advanced technology in detection of DNA protein binding sites so far. However,the existed analysis methods only use DNase-Seq data to recognize DNA protein binding sites.In this project, a novel idea is proposed to further recognize the kinds of these binding sites or the kinds of DNA proteins bound to these binding sites to acquire the whole information, which can be used to dynamically investigate real-time binding status of all kinds of DNA proteins in high resolution. Based on this idea, a novel analysis method would be researched, where through pre-processing of DNase-Seq data, whole inforamtion acquiring and isolating of DNA protein binding sites, by enhancing useful information in DNase-Seq data, binding status of all kinds of DNA proteins in whole genome would be analyzed, which are helpful in dynamically investigating real-time complicated regulation patterns of all kinds of DNA proteins in high resolution, and even the high-level biological regulation mechanisms.

英文关键词： nucleotide molecular information;gene sequencing;nucleotide detection;protein binding site