带界面结构的一体化仿生骨软骨支架的研制及修复骨软骨缺损的实验研究

项目名称： 带界面结构的一体化仿生骨软骨支架的研制及修复骨软骨缺损的实验研究

项目编号： No.31300798

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 赵艳红

作者单位： 天津医科大学

项目金额： 25万元

中文摘要： 关节骨-软骨联合损伤常见且修复困难，构建组织工程骨软骨复合体是目前非常有前景的治疗方法。鉴于骨软骨界面结构对隔离成骨和成软骨微环境的重要作用，制造带界面结构的一体化仿生骨软骨支架是目前研究的热点与难点。本项目拟采用丝素蛋白（SF）/软骨细胞外基质（CECM）材料,利用TIPS技术制备具有取向微孔结构的软骨支架部分；采用SF/纳米羟基磷灰石（n-HA）材料，利用"相分离/沥滤技术"制备具有高贯通性和孔隙率的骨支架部分；二者之间以致密的CECM/SF/n-HA层连接。将脂肪基质干细胞向成软骨、骨诱导分化后分别接种到一体化骨软骨支架相应部位，在体外培养后植入兔股骨髁负重区骨软骨联合缺损（以无界面结构支架为对照），通过组织学、生化成分及生物力学等指标检测骨软骨修复效果，重点观察骨软骨界面愈合情况，探讨界面结构对组织工程骨软骨组织修复的影响。本项目可为仿生构建理想的骨软骨复合体提供新材料和新策略。

中文关键词： 骨软骨；界面结构；丝素；组织工程；脂肪基质干细胞

英文摘要： Articular osteochondral injury is common, while it is diffcult to repair. Osteochondral tissue engineering is a newly but promising treatment opition. In view of the important role of the "bone-cartilage interface" structure to isolate the microenvironment of bone and cartilage, fabrication of integrated biomimetic osteochondral scaffold with bone-cartilage interface structure is currently a hot and difficult research. In this project, cartilage portion of osteochondral scaffold with oriented microporous structure would be fabricated with silk fibroin (SF)/cartilage extracellular matrix (CECM) composite materials by TIPS technique.Bone portion with high perforation and porosity would be prepared with SF/nanometer-hydroxyapatite (n-HA) material by "phase separation/leaching" technique, and compact CECM/SF/n-HA layer would be used to integrate cartilage and bone portion of the scaffold. In this study, the integrated osteochondral scaffolds combined with chondrogenic and osteogenic induced adipose-derived stem cells were cultured in vitro and implanated into osteochondral defects of high-load-bearing area of rabbit femoral condyle(the scaffold without interface structure for control). A morphologic, histological,biochemical,and biomechanical assessment of repair effects of osteochondral defects would be conducted.

英文关键词： Osteochondral；interface；silk fibrin；tissue engineering；ADSC