GTF2H5在小鼠胚胎早期发育中的功能和作用机制研究

项目名称： GTF2H5在小鼠胚胎早期发育中的功能和作用机制研究

项目编号： No.31301184

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 冯云

作者单位： 同济大学

项目金额： 25万元

中文摘要： 通过体外受精可以在培养条件下完整呈现早期胚胎发育的全部事件，这也是临床辅助生殖的基础。早期胚胎发育是多基因选择性时-空表达共同调控的复杂过程，最终导致母源性调控向合子性调控转变。研究这一过程中关键调控因子的功能和机制，可以更好地指导并改进人工辅助生殖技术。利用单细胞转录组测序及权重基因共表达关联性分析，研究早期胚胎发育过程的基因表达调控规律，我们已经发现了人和小鼠胚胎中均存在一个作用保守的关键调控因子GTF2H5，生物信息学分析表明它在8细胞期胚胎与线粒体相关基因的转录密切相关。本项目进一步深入研究GTF2H5在胚胎从受精卵向囊胚发育进程中的生物学功能以及对线粒体功能调控的作用，并分析其在基因表达调控中的作用机理。本项目的研究成果可以拓展辅助生殖技术的理论依据，找到一条能够提高IVF胚胎正常发育的有效途径。

中文关键词： 滋养外胚层；内细胞团；单细胞RNA测序；基因网络；

英文摘要： In vitro fertilization（IVF）can fulfill the entire early embryo events under culture condition, which is the major basis of assisited reproductive technology (ART). Mammalian preimplantation embryo development is a life processes including co-regulation of multi-gene selectively time-space expression, such as conversion of maternal regulation to the zygote regulation. However, embryo events are the outputs of the interplay among hundards of genes, some major genes play dominant function in specific embryo-stage and stay in the central network of genes. It is very important to make clear the biological function and the underlied mechanism of these central factors for clinical uses. Single-cell transcriptome sequencing and weighted gene correlation network analysis (WGCNA) points out GTF2H5 is one of the central factors, it conservedly participate in regulation of gene expression, especially the mitochondrial-related genes in mouse 8-cell embryos. In both human and mouse, GTF2H5 plays very important roles during the initiation of independent energitic supply in early embryo. This project will continue to study the biological role of GTF2H5 in the process from fertilized egg to blastocyte and analysize its mechanism in gene activities and mitochondrial function, we try to extend the theory basis of IVF and find an e

英文关键词： trophoectoderm；inner cell mass；single cell RNA-seq；gene network；