冷胁迫诱导柽柳ThCAP基因表达的分子机制研究

项目名称： 冷胁迫诱导柽柳ThCAP基因表达的分子机制研究

项目编号： No.31200510

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 林学

项目作者： 郭晓红

作者单位： 黑龙江八一农垦大学

项目金额： 23万元

中文摘要： 柽柳冷适应蛋白ThCAP基因（基因登陆号：AY587773）属于COR413基因家族的一个新基因，申请者前期研究结果表明其在植物抗冷胁迫过程中起到重要作用。本项目拟以克隆的ThCAP基因启动子为基础，通过启动子5'系列缺失技术鉴定出柽柳ThCAP基因启动子中冷响应的关键调控区域；采用酵母单杂交体系筛选出调控该区域的候选上游转录因子（TF），进一步通过凝胶阻滞试验验证TF的正确性；通过转基因技术在拟南芥中过表达TF，研究转基因植株的抗冷相关生理指标和基因表达分析，明确TF在低温胁迫条件下的生物学功能，从而获得冷胁迫诱导柽柳ThCAP基因表达的分子机制，为全面揭示COR413基因表达的调控机制奠定基础。

中文关键词： 柽柳；启动子；转录因子；COR413基因；

英文摘要： A new gene （GeneBank accession:AY587773）encoding cold acclimation protein, named ThCAP belongs to COR413 gene family. Our previous research showed that the ThCAP gene was involved in temperature response and played important roles in stress responses. In this study, The sequence of the ThCAP gene promoter was used as research base, and the key regulation region of ThCAP promoter to cold response was identified by its seried of 5'-deleted promoter technology. The upstream transcription factor（TF）which regulate the cold responsive region of ThCAP promoter was selected thought a yeast one-hybrid experiment, and identified by electrophoretic mobility shift assay（EMSA）. The over-expression vector of the TF gene was constructed and transformed into Arabidopsis to study its biological function according to cold-resistance physiological indicator and expression analysis of related gene.The molecular mechanism of ThCAP gene expression from Tamarix hispida in response to cold stress was obtained.These results will help to provide theoretical basis for revealing the expression regulation mechanism of COR413 gene.

英文关键词： Tamarix hispida；promoter；transcription factor；COR413；