酸菜发酵生态系统中细菌群落与代谢物组特征耦合机制

项目名称： 酸菜发酵生态系统中细菌群落与代谢物组特征耦合机制

项目编号： No.31300355

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 赵丹

作者单位： 黑龙江大学

项目金额： 26万元

中文摘要： 微生物群落与环境构成的生态系统是研究物种及其与环境相互作用机制的理想模型。代谢物是细胞对环境变化的终端响应，因此群落代谢物组是生态系统结构和功能的直接表征。本项目拟构建供试菌L. paracasei HD1.7异型乳酸发酵代谢网络，并以其为发酵剂构建的酸菜发酵生态系统为研究模型，采用稳定性同位素标记、核磁共振和液相-质谱联用等代谢组学技术，结合实时定量PCR和条码焦磷酸测序等基因组学技术，研究细菌群落数量、结构和代谢物组动态变化，探讨细菌群落演替和代谢物组变化的一般规律。通过比较分析供试菌单独发酵与研究模型、研究模型与对照模型中的细菌群落与代谢物组特征，探索（1）细菌群落与代谢物组特征耦合机制和（2）L. paracasei HD1.7对环境因素的代谢物组水平应答机制。本研究拓展了代谢组学在微生物生态学中的研究范畴，对酸菜发酵生产工艺的完善和质量检测体系的建立也具有实践指导意义。

中文关键词： 酸菜；副干酪乳杆菌；高通量测序；代谢组学；菌群演替

英文摘要： Microbial ecosystem, which is composed of microbial communities and the environment, is an ideal model to understand the interactions between species and the environment. Metabolites are the final response of cells to environmental changes. Therefore, metabolomes involved in communities directly represent the structure and function of the ecosystem. Firstly, this present work constructs the metabolic network of heterofermentative L. paracasei HD1.7. Secondly, this study takes the L. paracasei HD1.7 starter culture fermentation of pickle Chinese cabbage as research model as well as the natural fermentation of pickle Chinese cabbage as control model. Metabolomic techniques, including stable isotope labeling, nuclear magnetic resonance and chromatogram-mass spectrum, are applied to investigate the metabolomes features. Genomic techniques such as quantitative real-time PCR and barcoded pyrosequencing are adopted to research the variation of structure and quantity of bacterial communities. Subsequently, this research aims to figure out the dynamic regular pattern between bacterial communities and metabolomes. Finally, based on the comparative analysis on bacterial communities and motabolomes features (1) between L. paracasei HD1.7 and research model; (2) between research model and control model, the final goal is to

英文关键词： pickle Chinese cabbage；Lactobacillus paracasei；high throught sequencing；metabolomics；community succession