食品中纳米二氧化钛存在特征及膳食暴露风险评价

项目名称： 食品中纳米二氧化钛存在特征及膳食暴露风险评价

项目编号： No.31301474

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 王晓伟

作者单位： 北京市预防医学研究中心

项目金额： 25万元

中文摘要： 二氧化钛（TiO2）是一种重要的食品添加剂。由于其具有优良的增白与提色能力，近年来食品中TiO2非法添加现象逐渐增多。食品级TiO2本身无毒，但是添加TiO2的食品在处理过程中将产生纳米二氧化钛颗粒（NP-TiO2）。毒理学实验表明，NP-TiO2会造成孕鼠妊娠障碍，并且阻碍幼鼠神经发育，存在潜在的生物安全风险。本研究将考察我国食品中NP-TiO2 的存在情况，并对我国食品中NP-TiO2的膳食暴露风险进行评估。本研究将首先基于拉曼光谱技术，建立食品中TiO2的快速检测方法，对市售食品中TiO2添加情况进行筛查；基于浊点萃取技术，场流分级串联电感耦合等离子体质谱技术，建立食品中痕量NP-TiO2定性与定量分析方法。考察TiO2添加食品中NP-TiO2的粒径分布特征与浓度水平。根据食品消费情况等相关数据，建立NP-TiO2的膳食暴露模型，评估我国居民膳食中NP-TiO2的暴露水平。

中文关键词： 纳米二氧化钛；膳食暴露风险；儿童；单颗粒分析；拉曼光谱

英文摘要： Titanium dioxide (TiO2) is a common food additive. Due to its outstanding properties, the illegally addition of TiO2 in food was gradually increased in recent years. Food grade TiO2 is non-toxic, but it will generate titanium dioxide nano-particles (NP-TiO2) during the manufacture process. According to the toxicology experiments, the NP-TiO2 showed toxicity toward animal. The NP-TiO2 causes pregnancy complications when injected intravenously into pregnant mice. Mice treated with NP-TiO2 had smaller uteri and smaller fetuses than untreated. The aim of this study is to establish the qualitative and quantitative methods, to teat the presence of TiO2 and NP-TiO2 in food, and inspect the concentration and particle size of NP-TiO2, finally carry on the dietary exposure assessment. Using Raman spectroscopy, rapid detection method will at first established to screen the presence of TiO2 in food. Then the NP-TiO2 in food will be extracted with cloud point extraction, and determined by field-flow fractionation coupled with inductively coupled plasma mass spectrometry. After that,the particle size distribution and concentration level of NP-TiO2 in foods will be analyzed. Finally, based on the food consumption data, the dietary exposure model of NP-TiO2 will be eatablished, and the risk of dietary exposure for public will

英文关键词： nanoscale titanium dioxide；dietary exposure risk；children；single particle analysis；Raman spectroscopy