从新疆维哈2型糖尿病人粪便分离的关键菌群致小鼠2型糖尿病的作用及机制

项目名称： 从新疆维哈2型糖尿病人粪便分离的关键菌群致小鼠2型糖尿病的作用及机制

项目编号： No.81460562

项目类型： 地区科学基金项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 李琳琳

作者单位： 新疆医科大学

项目金额： 45万元

中文摘要： 最新研究表明肠道菌群与2型糖尿病（T2DM）密切相关，但目前还缺少足够证据来说明肠道菌群与T2DM发生的因果关系。本课题组前期研究表明：新疆哈族T2DM发病率低于维族（8.16%），仅为1.47%，对其原因探究，发现两民族正常糖耐量人群（NGT）与T2DM人群差异肠道菌群特征：基于Illumina16S rDNA 高变区测序技术，哈族T2DM组粪便脱硫弧菌、韦荣球菌、气单胞菌丰度显著升高，对肠道有益的丁酸(GC-MS检测)明显下降；维族T2DM组红椿菌菌群丰度显著升高；与哈族NGT组相比，维族NGT组芽孢杆菌，梭杆菌菌群丰度显著升高；故假设维、哈两民族T2DM人群差异肠道菌群可能对T2DM发生发展有一定作用。拟从维哈人群粪便内培养分离鉴定上述差异菌，将其回接到正常、无菌或低菌动物，复制2型糖尿病动物模型，探索差异菌对T2DM的作用及机制，为肠道菌群与T2DM之间的因果问题提供明确的佐证

中文关键词： 新疆维哈民族；关键肠道菌群；2型糖尿病；作用机制

英文摘要： The latest research shows that the gut flora is closely associated with type 2 diabetes mellitus (T2DM). But there is not enough evidence to show a causal relationship between gut flora and T2DM in the world. Our previous study showed that the incidence of T2DM in Xinjiang Kazakhs was far lower than the Uighurs (8.16%), only 1.47%. To explore its causes, we found the characteristics of intestinal flora in normal glucose tolerance (NGT) and T2DM population. Based on Illumina 16S rDNA hypervariable region sequencing technology, the abundance of Desulfovibrionaceae, Veillonellaceae, Aeromonadaceae of feces in Kazakhs with T2DM group were significantly increased, but the content of butyric acid from GC-MS was significantly decreased while butyric acid is beneficial for intestinal health. The abundance of Coriobacteriaceae in Uighurs with T2DM were significantly higher. Compared with the NGT group in Kazakhs, the abundance of Bacillaceae, Fusobacteriaceae were significantly higher in Uighurs NGT group. Therefore, we assume that the differences of intestinal flora of the two ethnics may have a role in the development of T2DM. We intend to isolate、culture and identify these key fecal flora from the Kazakhs and Uighurs . Then they will be tied back to normal, sterile or low bacteria animals to copy animal models of T2DM. We will explore the role and mechanism of T2DM arising from different intestinal flora, to provide more definitive evidence for the causes and consequences between intestinal flora and T2DM, and to provide new ideas for early prevention and effective treatment of T2DM..

英文关键词： Uigurs and Kzarkhs;key gut flora;type 2 diabetes;mechanisms