N-乙酰半乳糖胺代谢通路在高致病性猪链球菌中的作用及其调控机制研究

项目名称： N-乙酰半乳糖胺代谢通路在高致病性猪链球菌中的作用及其调控机制研究

项目编号： No.81501725

项目类型： 青年科学基金项目

立项/批准年度： 2016

项目学科： 医药、卫生

项目作者： 胡丹

作者单位： 中国人民解放军东部战区疾病预防控制中心

项目金额： 18万元

中文摘要： N-乙酰半乳糖胺（GalNac）是细菌碳源的重要提供者，其中磷酸转移酶系统（PTS）是细菌碳源代谢与毒力相关联的纽带。课题组一直致力于重要病原菌2型猪链球菌（SS2）致病机理的研究，率先完成了SS2中国强毒株的全基因组测序及功能注释, 但尚不清楚中国高致病性SS2是否也存在参与GalNac转运的PTS系统，它又是在怎样的调控网络下发挥作用也有待深入认识。课题组前期对中国强毒株的基因组分析发现了27个编码PTS组分基因，功能预测筛选到一个可能参与GalNac的转运代谢的aga基因簇。本项目拟在前期研究基础上进一步鉴定GalNac转运基因簇PTS的转录特征，通过基因敲除的方法研究GalNac代谢通路在SS2中的作用，并利用EMSA等手段解析该通路的调控机制及效应分子。研究结果不仅有助于阐明SS2 GalNac代谢通路的分子调控机制，还将进一步深化对国内SS2高致病性的分子机理的认识。

中文关键词： 2型猪链球菌；磷酸转移酶系统；N-乙酰半乳糖胺；致病机制；调控网络

英文摘要： N-acetyl-galactosamine (GalNac) is the important provider of bacterial carbon source and the phosphotransferase system (PTS) is the link between bacterial carbon source metabolism and virulence. In the recent 10 years, our group has been committed to the pathogenesis of streptococcus suis type 2 (SS2) which is the severe Zoonosis, and the first to complete whole genome sequencing and functional annotation about the SS2 China's a virulent strain, but it is still unclear if SS2 is involve in PTS system of GalNac and what is a regulation network to be working. Our group further to analysis the genome of China's virulent strain 05ZYH33, and found 27 genes which is coding PTS component, then filter to an AGA gene cluster which is possible involvement in metabolism of GalNac by function prediction. In this project, we intended to identify the characteristic of GalNac PTS transcription trans-gene cluster on the basis of previous studies , compare the difference of pathogenicity between wild strains and target gene deletion mutants of SS2 by homologous recombination methods and assess the GalNac metabolic pathway molecular regulation mechanism ,which not only help to clarify SS2 GalNac metabolic pathway but also to make the molecular mechanism of SS2 highly pathogenic more clear.

英文关键词： streptococcus suis; phosphotransferase system;N-acetyl-galactosamine;Pathogenic mechanism;regulation