从功能成像- Rho GTPases信号研究补阳还五汤迭加丰富环境刺激对神经干细胞定向迁移的调控

项目名称： 从功能成像- Rho GTPases信号研究补阳还五汤迭加丰富环境刺激对神经干细胞定向迁移的调控

项目编号： No.81473745

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 医药、卫生

项目作者： 赵晖

作者单位： 首都医科大学

项目金额： 74万元

中文摘要： 探索促进内源性神经干细胞增殖、定向迁移的新方法是卒中康复研究的热点和难点。国内外研究证实存在多个干预因子的环境（丰富环境）能促进神经发生，是有效、低风险、低成本的康复手段。补阳还五汤迭加丰富环境刺激可促进内源性神经干细胞向缺血损伤区迁移是前一项国家自然基金项目研究中获得的重要结果。在此基础上，本项目拟采用PET脑代谢成像技术确定补阳还五汤、丰富环境刺激及二者联合作用的脑功能区，通过MRI技术示踪内源性神经干细胞迁移，从功能影像水平动态观察补阳还五汤迭加丰富环境刺激对神经干细胞定向迁移的调控模式。并进一步从细胞迁移的分子开关Rho GTP酶信号通路揭示其调控内源性神经干细胞定向迁移的关键环节。将整体水平的脑功能变化与细胞应答反应的信号分子相结合，研究补阳还五汤迭加丰富环境刺激对神经干细胞定向迁移的调控机制，为中药治疗和丰富环境刺激在卒中康复中有机结合运用提供强有力的理论支持。

中文关键词： 缺血性脑卒中；康复训练；作用机制

英文摘要： It is a very important but difficult question in the field of stroke rehabilitation how to promote the proliferation of endogenous neural stem cells and modulate its targeted migration.Domestic and foreign research confirmed that enriched environment including multiple interference factors of environment could promote neurogenesis and enriched environment was an effective and low risk rehabilitation means.Invigorating yang for recuperation decoction combined with enriched environmental stimulus could promote endogenous neural stem cells migrating to ischemic region which was the important result of previous project National Natural Science Foundation of China. Based on previous research, the brain functional region of invigorating yang for recuperation decoction and enriched environment stimulus as well as the combination will be confirmed by positron emission tomography (PET) which is a kind of cerebral metabolic imaging technology.The targeted migration and distribution of endogenous neural stem cells will be traced by MRI in order to observe the modulation pattern of invigorating yang for recuperation decoction combined with enriched environmental stimulus on targeted migration of newborn neurons. Moreover the key process of invigorating yang for recuperation decoction combined with enriched environmental stimulus on targeted migration of newborn neurons will be explained by the Rho GTP enzyme which is the molecular switch of cell migration. The characteristic of our research is to explore the mechansim of invigorating yang for recuperation decoction combined with enriched environmental stimulus from the integral level -the change of brain function and the molecular level -signal molecules of cell response .Our objective is to provide theoretical evidences forcefully for application of the Chinese herb combined with enriched environmental stimulus environment correctly and timely.

英文关键词： cerebral ischemic stroke;rehabilitation training;mechanism of action