低强度超声波信号对ORNM的防治作用及机理研究

项目名称： 低强度超声波信号对ORNM的防治作用及机理研究

项目编号： No.61471384

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 无线电电子学、电信技术

项目作者： 吴高义

作者单位： 中国人民解放军联勤保障部队第九六〇医院

项目金额： 87万元

中文摘要： 颌骨放射性骨坏死（ORNM）是头颈部恶性肿瘤放疗后的严重并发症,严重影响患者的生活质量。随着近年来放疗以及放-化疗联合治疗的广泛开展，ORNM已成为头颈部恶性肿瘤治疗中亟需解决的严重问题，目前尚无有效手段进行防治。本课题组前期研究发现，低强度超声波能有效促进ORNM的创面愈合，骨损伤区域的成骨细胞、骨小梁数量等明显增加，同时对血管损伤也具有一定的修复作用。然而，何种超声波信号能达到最佳的ORNM损伤修复效果？其具体的作用机制和调控机理如何尚不清楚。因此，本项目拟在前期研究的基础上，通过综合应用Micro-CT、分子生物学等技术，筛选出防治ORNM的最佳超声波信号作用方式；明确超声波信号对放射线损伤的骨和血管等组织修复作用，揭示其可能的作用机制。通过本研究可望阐明超声波信号防治放射性骨坏死的新机制，为超声治疗学开辟新的应用领域，具有重要的理论意义和潜在应用价值。

中文关键词： 超声波热治疗；放射线；医学电生理检测；颌骨；放射性骨坏死

英文摘要： Osteoradionecrosis of mandible (ORNM) is a common severe complication of radiotherapy on malignant head and neck carcinoma that undermines patient's life quality. Its morbidity mounts with the increase of radiotherapy dosage. ORNM has become a challenge of tumor therapeutics in recent years as the combined treatment of radiotherapy and chemotherapy catches on. Effective prolonged management of ORNM is in demand. It is observed lately that low intensity ultrasound accelerates the recovery of fractures with healing difficulty. Based on this finding, our team launched a pre-examination on small sample of animal and found that low intensity ultrasound assisted the healing of ORNM,but result was not statistically significant due to small sample size. Therefore, this research is aimed at identifying the protective effect of low intensity ultrasound on ORNM and its mechanism in a quantitative manner based on the pre-examination result, namely to systematically observe ultramicro pathological changes in mandible tissue after radiotherapy and changes of metabolism, apoptosis, proliferation and gene expression after ultrasonic therapy. Research methods involved physiology, pathology, molecular biology and imaging. The results are conducive to the establishment of biological theory of ultrasound's effect on mandible after radiotherapy, simple and inexpensive management of the disease and the improved availability and effect of radiotherapy.

英文关键词： Ultrasound Heat Treatment;radioactive ray;Electrophysiological detection of medici;Mandible;osteoradionecrosis