猪骨骼肌发育相关新转录因子MASTR的靶基因及其调控通路研究

项目名称： 猪骨骼肌发育相关新转录因子MASTR的靶基因及其调控通路研究

项目编号： No.U1304325

项目类型： 联合基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 韩雪蕾

作者单位： 河南农业大学

项目金额： 31万元

中文摘要： 寻找鉴定与猪骨骼肌的生长发育相关的新基因，并对其调控机制和功能进行研究，对了解猪骨骼肌生长发育的分子调控机制，加速猪优质、高产新品种（系）的培育具有重要意义。本项目拟以前期对骨骼肌发育中新转录因子MASTR的研究为基础，利用染色质免疫共沉淀-测序（ChIP-seq）技术钓取猪转录因子MASTR的靶序列，通过荧光素酶报告系统和EMSA等方法对钓取的靶基因进行生物学验证，并在细胞水平上对猪MASTR基因进行超表达、抑制表达进而检测靶基因的表达量的变化，挖掘影响猪骨骼肌生长发育靶基因及其重要信号通路，同时结合靶基因SNPs扫描和性状关联分析结果，综合推测MASTR基因在骨骼肌生长发育过程中的作用。研究结果为揭示猪MASTR基因的功能及其调控机制、进一步完善猪骨骼肌生长发育的调控网络、靶基因更深入的功能研究奠定基础，同时为猪分子育种提供新的遗传素材，有重要的学术价值与应用前景。

中文关键词： 猪；骨骼肌；调控机制；ChIP-seq技术；

英文摘要： It is important to understanding of molecular regulation mechanism of skeletal muscle and accelerated growth and development of the pig quality, cultivation of high-yield varieties (lines) by identified and studied its regulation mechanism and function of the new gene in skeletal muscle development. The project intends to catch the target sequence of transcription factor MASTR using the ChIP-seq technical basis on the previous study results in skeletal muscle of porcine. And then verified of the select terget gene using Luciferase reporter system and EMSA, and also detected the expression levels of target gene by over-expression and inhibition of expression MASTR in cell level, finally excavate the important gene and its signaling pathway in the development of skeletal muscle. And then speculated the role in skeletal muscle growth and development process of MASTR by combined the SNPs scan and trait association analysis results of target gene. The results establish the foundation of reveal the function and regulation mechanism, and further complete the regulation network of skeletal muscle and the more in-depth study function of target gene. At the same time, provid the new genetic material to the molecular breeding of pigs. Therefore, the project have important academic value and application prospects.

英文关键词： pig；sketetal muscle；rgulatory mechanism；ChIP-seq technology；