海带多糖干预动脉粥样硬化发生的结构基础与作用机理

项目名称： 海带多糖干预动脉粥样硬化发生的结构基础与作用机理

项目编号： No.31271814

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 查学强

作者单位： 合肥工业大学

项目金额： 86万元

中文摘要： 动脉粥样硬化(AS)是心脑血管疾病发生的主要病理基础，严重威胁着人类健康和生活质量，但至今无有效的治疗措施，寻找有效的预防AS发生发展的功能因子，对解决心脑血管疾病的防治问题具有十分重要的意义。我们前期的研究发现，多糖是海带发挥抗AS功能的活性化学成分，且不同分子量多糖组分的活性差异较大。本项目将以此为基础，以高脂饲料诱导LDLr-/-小鼠动脉粥样硬化为活性评价模型，从海带中提取分离纯化活性多糖单一组分，解析其化学结构；在此基础上，借助多糖分子修饰技术，通过研究抗AS活性多糖的构效关系，阐明多糖抗AS功能所需的结构基础；以MAPK、JAK-STAT和NF-κB信号转导通路为切入点，通过研究多糖对信号通路关键蛋白的调控作用，明确多糖干预AS病变发生的信号转导机制，探讨多糖干预AS发生的作用机理。研究成果将为深入研究多糖抗AS的作用机理和目的性筛选与设计高抗AS活性多糖类功能因子奠定理论基础。

中文关键词： 海带；多糖；动脉粥样硬化；化学结构；构效关系

英文摘要： Atherosclerosis （AS） is the primary cause of cardiovascular and cerebrovascular diseases, both of which are the two causes of death around the world and thus seriously affect the human health and life quality. However, there is no effective solution to treat this disease until today. Recently, there is considerable interest in identifying preventive factors that may delay the onset or progression of atherosclerosis, which will have great significance for the prevention of cardiovascular and cerebrovascular diseases. Our previous experimental findings revealed that polysaccharides are the bioactive compounds in Laminaria japonica for preventing onset and progression of atherosclerosis, and the differences were obviously observed in pharmacological activities among the polysaccharide fractions with different molecular weight. Accordingly, this project aims to study the structural basis and mechanism of anti-atherosclerosis of polysaccharides from Laminaria japonica (LP). Firstly, the crude polysaccharides will be extracted from the raw materials of Laminaria japonica, and the active and homogenous polysaccharide fractions will be obtained from the crude LP after separation and purification. At the same time, the atherosclerosis model of LDL receptor-dificient (LDLr-/-) mice induced by high fat diet will be applied

英文关键词： Laminaria japonica；Polysaccharide；Atherosclerosis；Chemical structure；Structure-activity relationship