硝酸盐对中亚滨藜耐盐胁迫的信号调节机制研究

项目名称： 硝酸盐对中亚滨藜耐盐胁迫的信号调节机制研究

项目编号： No.31272239

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 徐进

作者单位： 中国科学院遗传与发育生物学研究所农业资源研究中心

项目金额： 80万元

中文摘要： 中亚滨藜是一种典型的盐生植物,具有盐囊泡结构和二型性种子等特殊的耐盐胁迫的生理生态特征。研究发现，中亚滨藜二型种子的幼苗耐盐性与其吸收硝态氮 (NO3-) 和一氧化氮 (NO) 水平的差异呈正相关。然而NO3-的积累与NO信号之间的相互关系，以及这种关系对植株耐盐性的影响，则还不明确。因此，本项目拟研究中亚滨藜两型种子幼苗在盐胁迫下对NO3-累积和同化过程与NO信号之间的关系，以及NO对NO3-吸收和代谢的反馈调节机制，鉴定参与这一调控过程的相关基因及信号途径。同时，构建NO3-转运蛋白沉默的转基因材料，验证硝态氮在耐盐胁迫中的功能。本研究对于揭示极端生境植物的耐盐机制以及耐盐植物基因工程具有重要意义；同时对盐渍化土壤中如何通过调控氮肥形态，提高作物的耐盐性提供重要借鉴。

中文关键词： 中亚滨藜；硝酸盐；一氧化氮；盐胁迫；转录调节

英文摘要： Atriplex centralasiatica is a herbaceous salt-secreting halophyte with particular mechanisms of salt tolerance, such as salt bladder and seed dimorphism. In previous study, we found that the salt tolerance of the seedlings derived from dimorphic seeds are correlated with the accumulation of nitrate (NO3-) and the production of nitric oxide (NO) in plants. However, the interaction between NO3- accumulation and NO level, and the effects of the interaction on salt tolerance are not fully understood. The main aim of this study is to elucidate the roles of NO3- accumulation and assimilation in modulating NO signaling in A. centralasiatica in response to salt stress and the feedback modulation of NO on NO3- accumulation, and identify the key genes and signaling transduction pathways that involved in these processes. Further confirmation of the roles of NO3- signaling in salt tolerance in plants is performed by studying the NTR1.1-RNAi transgenic A. centralasiatica plants. This study would provide an explanation for the role of nitrate-involved NO production in the plant growth and adaptation to salt stress, and benefit future work to reveal the molecular mechanisms of nitrate in modulating plant salt tolerance. Such an understanding is helpful for the breeding and cultivation of crop in saline soil, and provides insig

英文关键词： Atriplex centralasiatica；nitrate；nitric oxide；salt stress；transcriptional regulation