基于代谢组学探究牛蒡叶抑制食源性致病菌生物膜的活性成分与机理

项目名称： 基于代谢组学探究牛蒡叶抑制食源性致病菌生物膜的活性成分与机理

项目编号： No.31201433

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品配料及其他

项目作者： 娄在祥

作者单位： 江南大学

项目金额： 24万元

中文摘要： 有害菌生物膜是食品的顽固污染源，易引发大规模食物中毒事件。目前，植物成分对生物膜的抑制效果引起了学者的广泛关注，但是植物组分抑制食源性致病菌生物膜的有效成分及其机理尚缺乏系统研究。 前期研究结果表明牛蒡叶提取物对食源性致病菌生物膜具有显著的抑制作用。本课题拟采用UPLC-MS/MS与NMR技术分别对牛蒡叶提取物和食源性致病菌生物膜小分子代谢组进行测定，建立分析方法。利用牛蒡叶提取物抑制食源性致病菌生物膜，采用UPLC-MS/MS、NMR技术系统分析牛蒡叶提取物及提取物抑制前后细菌生物膜代谢组的动态变化，结合化学计量学、生物信息学等手段筛选出抑制生物膜的活性化合物，探索一种筛选生物膜抑制成分的新途径。通过分析牛蒡叶组分抑制过程中食源性致病菌生物膜代谢组的动态变化，探讨牛蒡叶对生物膜代谢及生理生化状态的影响，揭示牛蒡叶抑制生物膜的动态过程和作用靶点，以期为有害菌生物膜的控制提供新思路与新靶点。

中文关键词： 生物膜；代谢组学；活性成分；机理；牛蒡叶

英文摘要： As a stubborn source of pollution in food, bacterial biofilm can easily cause large-scale food poisoning. At present, the inhibitory effect of plant components on bacterial biofilms has gained more and more attention among scholars. But the chemical composition and mechanism of the anti-biofilm plant fractions still lacked systematic research. Our preliminary experiments indicated that burdock leaf extract exhibited significant inhibition effect on foodborne bacterial biofilm. In this research, burdock leaf extract and the metabolomes of biofilms will be analyze using UPLC-MS/MS and NMR, and a determination method will be established. Using burdock leaf extract to inhibit biofilm, burdock leaf fractions and the metabolomes of biofilms before and after inhibition by the fractions of burdock leaf will be dynamically analyzed using UPLC-MS/MS and NMR. On this basis, through chemometrics, bioinformatics and other means, find the anti-biofilm compounds and establish a new method for the screening of anti-biofilm compounds. Based on the dynamical analysis of biofilm metabolomes, evaluate the effects of burdock leaf components on the metabolism, physiology and biochemistry of biofilm, revealing the inhibiting targets and dynamic process against bacterial biofilms. It is hoped that this research could provide new ide

英文关键词： biofilm；Metabolomics；effective compounds；mechanism；burdock leaf