miR-23调控疲劳型亚健康骨骼肌线粒体再生的机制及维康颗粒的干预研究

项目名称： miR-23调控疲劳型亚健康骨骼肌线粒体再生的机制及维康颗粒的干预研究

项目编号： No.81202736

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学八处

项目作者： 聂晓莉

作者单位： 南方医科大学

项目金额： 23万元

中文摘要： 流行病学调查显示，疲劳型亚健康严重影响人群健康，然而目前对它的发生机制知之甚少，临床上也缺乏公认的治疗药物。前期研究发现疲劳型亚健康存在能量代谢紊乱和线粒体功能障碍，但具体机制不清。miR-23可通过调控下游AMPK-PGC1α-SIRT1信号通路影响线粒体功能。本项目拟在前期研究的基础上，以疲劳型亚健康大鼠模型和骨骼肌细胞线粒体为研究对象，利用miRNA慢病毒转染、qRT-PCR、Western blo等技术方法，研究miR-23表达水平对骨骼肌线粒体再生功能指标及细胞AMPK-PGC1α-SIRT1信号通路的影响，阐明miR-23调控疲劳型亚健康线粒体再生的分子机制。同时观察中药维康颗粒对miR-23调控线粒体再生通路的影响，明确维康颗粒防治疲劳型亚健康的药物作用靶点及机制。本项目不仅是对疲劳型亚健康发生机制的新探索，也是对我们前期工作"疲劳型亚健康能量代谢紊乱"上游机制的进一步探讨。

中文关键词： 疲劳型亚健康；小分子RNA；中药；信号通路；线粒体功能障碍

英文摘要： The fatigue-type sub-health affects human health seriously. The clinical prevention and treatment is difficult for the reason of the mechanism about it is not clear. Our previous research showed that the fatigue-type sub-health existing abnormal structure and dysfunction of mitochondria. We also found that the miR-23 can regulate the mitochondria biogenesis and alleviate the fatigue-type sub-health. But we don't know how it works. We research how the different miR-23 expression levels affect the regeneration index of skeletal muscle mitochondria and the pathway of cell AMPK-PGC1α-SIRT1 signal transfer based on our preliminary studies. Those works also could elucidate how miR-23 regulates the fatigue-type sub-health mitochondrial regeneration. On the other hand, we observe how Weikang granules influence the miR-23 regulation of mitochondrial regeneration pathway, which is a National Invention Patent Chinese medicine of anti-fatigue-type sub-health. And make clear the drug targets and mechanism of Weikang granules' effect on fatigue-type sub-health at the same time. In short, this project is not only a new exploration of the mechanism of fatigue-type sub-health, but also to further explore the upstream mechanism of our previous work of "the fatigue-type sub-health energy metabolism disorders". This project also ha

英文关键词： sub-health fatigue；microRNA；Traditional Chinese Medicine；signal pathway；mitochondria biogenesis