产毒水华蓝藻胁迫下铜锈环棱螺的代谢响应机制研究

项目名称： 产毒水华蓝藻胁迫下铜锈环棱螺的代谢响应机制研究

项目编号： No.31302192

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 农业科学

项目作者： 朱津永

作者单位： 宁波大学

项目金额： 23万元

中文摘要： 本项目针对水华蓝藻及其毒素对水生生物的生态毒理学研究中尚未明晰的理论问题- - 动物对微囊藻毒素（MCs）的分子响应机制，开展无脊椎动物体内总MCs负荷（结合态和非结合态MCs）、代谢物动态及代谢响应机制研究。以课题组现有淡水腹足纲模型动物,铜锈环棱螺为研究对象，在投喂产毒铜绿微囊藻后监测两态MCs在螺两种组织中的含量变化。在此基础上采用基于核磁共振的代谢组学技术，比较分析螺在投喂不同产毒特性铜绿微囊藻后其内源性代谢产物的差异，研究代谢组随时间的动态变化模式。旨在揭示螺类在受到MCs 胁迫时的代谢响应机制及相关的代谢产物，同时明晰不同组织内两态MCs的动态分布情况。丰富腹足纲动物与水华蓝藻间的相互作用的研究成果，为筛选理想的生物监测物种提供理论依据。

中文关键词： 铜锈环棱螺；微囊藻毒素；代谢组学；核磁共振；铜绿微囊藻

英文摘要： The theoretical problem in ecotoxicological studies of bloom-forming cyanobacteria and their toxins to aquatic organisms - - molecular response mechanism of animal to microcystins (MCs) is not yet clear. In order to answer the above question, this subject will be carried out the research on the accumulation of total MCs (bound and free MCs), the dynamic of metabolites and metabolic response mechanism in vertebrates. based on the animal model of Gastropoda Bellamya aeruginosa from pilot study, the content of the two-state MCs in two tissues of snail will be monitored after fed with toxic Microcystis aeruginosa. Using NMR-based metabolomics technology, the differences of the endogenous metabolites in snails between two treatments fed with the different toxigenic characteristics Microcystis aeruginosa will be analyzed, the dynamic pattern of metabonomics over time will be studied. metabolic response mechanism of snails to the MCs stress and related metabolites will be revealed, and the dynamic distribution of the two-state MCs in different tissues will be cleared at the same time. The results are expected to further enrich the achievement of interaction between bloom-forming cyanobatrica and gastropods, as well as providing a theoretical basis for screening the ideal species of biomonitor.

英文关键词： Bellamya aeruginosa；Microcystin；Metabolomic；Nuclear Magnetic Resonance；Microcystis aeruginosa