酶催化原位生成共反应试剂增强电致化学发光的超灵敏适体传感器研究

项目名称： 酶催化原位生成共反应试剂增强电致化学发光的超灵敏适体传感器研究

项目编号： No.21275119

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 袁若

作者单位： 西南大学

项目金额： 80万元

中文摘要： 本项目拟以肿瘤和心血管疾病相关的血清标志物为检测目标蛋白，利用酶催化原位生成能显著增强新型功能化钌配合物电致化学发光的高效及稳定的共反应试剂，构建超灵敏电致化学发光适体传感器实现疾病相关血清标志物（如恶性肿瘤标志物：SHP2、TNF；心血管疾病标志物：VEGF、PPGF）的痕量检测。以超分子水胶/纳米粒子功能化石墨烯或C60纳米复合材料构建生物兼容性好、传导能力强和背景信号低的适体Ⅰ固载基质，采用金属纳米粒子功能化石墨烯、碳纳米管和树枝状聚合物等复合纳米材料固载适体Ⅱ、酶及钌配合物，利用双适体夹心反应模式，结合酶催化原位生成与功能化钌配合物在同一分子内或分子间的共反应试剂（如L-高半胱氨酸、青霉噻唑等），显著增强钌配合物电致化学发光强度和稳定性，构建新型超灵敏电致化学发光适体传感器，旨在建立一种特异性强、灵敏度高、成本低廉及检测快速的血清学诊断疾病的新方法，提高疾病诊断效率。

中文关键词： 电致化学发光；生物传感器；纳米材料；血清疾病标志物；原位生成共反应试剂

英文摘要： This project intends to construct the ultrasensitive electrochemiluminescence aptasensors based on in situ produced coreactants by enzymatic reaction, which could significantly enhance electrochemiluminescence efficiency and stability of the novel functionalized ruthenium complexes, for the trace determination of tumor and cardiovascular disease related serum markers (such as malignant tumor markers: SHP2, TNF; cardiovascular disease markers: VEGF, PPGF). The supramolecular hydrogel/different nanoparticles functionalized graphene or C60 nanocomposites with good biocompatibility, conductive ability and low background signal are used as the matrix of primary aptamer. Some nanocomposites, including metal nanoparticles functionalized graphene, carbon nanotubes and dendrimer polymer et al., are used as the carriers of secondary aptamer, enzyme and ruthenium complexes. With the double aptamer-based sandwich assay, the novel ultrasensitive electrochemiluminescence aptasensors could be constructed by the introducing of the in situ produced electrochemiluminescence coreactants (such as L-homocysteine, Penicilloyl salt etc.) by enzymatic reaction, which coexisted intra or inter of functionalized ruthenium complexes to significantly enhance electrochemiluminescence intensity and stability of the ruthenium complexes. In con

英文关键词： electrochemilumicescence；biosensor；nanocomposites；serum disease markers；in situ produced coreactant