能源作物荻在盐胁迫下的Na、K离子调控机理及燃烧值变化研究

项目名称： 能源作物荻在盐胁迫下的Na、K离子调控机理及燃烧值变化研究

项目编号： No.31201262

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 食品科学、农学基础与作物学

项目作者： 宗俊勤

作者单位： 江苏省中国科学院植物研究所

项目金额： 21万元

中文摘要： 生物质能源产业的发展和盐碱地开发利用均增加了对抗盐生物质能源植物的需求。荻是起源于我国的优良能源植物，具有较强的抗性，在盐渍化土壤上有较大的发展潜力。离子毒害（Na+）和相关重要营养元素（K+）的缺乏是盐胁迫对植物的主要伤害，荻在盐胁迫下的调控机制并不明确。本研究拟在已对130余份荻资源抗盐性进行鉴定的基础上，通过对荻不同抗盐类型材料在盐胁迫下的Na+、K+选择吸收、运输、分布以及植株蒸腾变化进行研究，从而明确其Na+、K+调控的生理机理；并基于cDNA-AFLP技术，对极端类型材料在盐胁迫下Na+、K+离子调控相关的基因表达状况进行研究，从分子层面分析其抗盐机理；同时研究在盐胁迫下不同调控措施对抗性材料地上部分燃烧值的影响，明确盐胁迫下提高荻燃烧值的调控措施。本项目研究将对荻抗盐性的遗传改良、抗盐基因的克隆以及盐胁迫下荻的栽培调控均具有重要的理论意义。

中文关键词： 荻；盐胁迫；离子调控；基因差异表达；热值

英文摘要： The need for salt tolerance biofuel plants increase due to both the development of biomass energy industry and the saline soil utilization. Miscanthus sacchariflora is a choiceness biomass energy plant originated in China. It is also a salt tolerance plant and has extensive future as a biofuel plant in saline soil. Na+ toxicity and K+ imbalance are the main injury to plant under the salinity stress, but the mechanism of Na+ and K+ regulation was unclear in M.sacchariflora. The salt tolerance genotypes, intermediate genotypes and the salt sensitive genotypes of M. sacchariflora are selected based on the salt tolerance evaluation of more than 130 genotypes. The physiological mechanism of Na+ and K+ regulation will be discussed by means of Na+ and K+ selective absorption, transportation, distribution and transpiration regulation by stoma using different salt tolerance genotypes of M. sacchariflora. The profile of induced genes concerned Na+ and K+ regulation under salinity stress will be analyzed by cNDA-AFLP technic using the different salt tolerance genotypes. At the same time, the combustion characteristics regulation of the salt tolerance genotypes will be studied by analyzing the calorific value variation under the salt stress condition. The study will provide theoretical foundation for genetic improvement of

英文关键词： Miscanthus sacchariflora；Salinity stress；Ion regulation；Gene expression；Calorific value