单粒子拉曼读出的编码微球对宫颈癌高危病毒基因组的同步检测

项目名称： 单粒子拉曼读出的编码微球对宫颈癌高危病毒基因组的同步检测

项目编号： No.61205152

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 信息四处

项目作者： 刘仁勇

作者单位： 中国科学院合肥物质科学研究院

项目金额： 28万元

中文摘要： 高危型人乳头瘤病毒（HPV）感染是宫颈癌早期诊断的直接指标，临床上急需对不同基因型别HPV进行同步鉴定和检测的快速简便方法。本项目拟探索单粒子拉曼读出和编码的方法实现不同基因型别HPV的同步检测：（1）设计Au和Ag复合多层壳空心纳米粒子和拉曼标签分子在壳层间的组装方法，通过多层壳厚度和间隙的调控，阐明电磁耦合和热点增强之间的关系，合成出具有高密度热点、强信号输出且强度一致的单粒子拉曼读出纳米标签；（2）探索拉曼活性物质对复合多层壳空心纳米粒子进行编码及寡核苷酸修饰方法，验证拉曼纳米标签对目标HPV的选择性和敏感性及标签信号输出的一致性；（3）设计基于磁性粒子选择性分离的"磁性固相载体-HPV核酸-标记探针"三明治检测结构，通过编码分子的指纹识别实现对不同基因型别HPV的同步检测，建立拉曼编码信号与HPV的定性定量对应关系及其识别模式。本项目的预期成果将为宫颈癌的早期诊断找到一条新的途径。

中文关键词： 单粒子拉曼；可控组装；检测；成像；

英文摘要： High-risk human papillomavirus (HPV) infection is the direct index of the early diagnosis of cervical cancer, it is urgent to develop a rapid and simple method for the simultaneous identification and detection of different types of HPV in clinical practice. In this program, we'll explore single-particle Raman readout and the encoded method to achieve the simultaneous detection of different types of HPV: (1) designing Au and Ag multilayered shell nanostructures with hollow interiors and the method for assembling the Raman tag molecules at the interlayers, explaining the relationship of electromagnetic coupling and the hot-spot enhancement by controlling the thickness and the gap of the multilayered shells, preparing the nanotags with highly dense hot spots, strong and uniform single-particle Raman readout; (2) exploring the method for the encoding of multilayered shell nanoparticles with Raman-active molecules and the modification of oligonucleotide sequences, and verifying the selectivity and sensitivity to the target HPV and the uniform readout of the nanotags; (3) designing the sandwiched structures of magnetic immobilization capture-HPV nucleic acid-labeled nanoprobe based on the selective separation of magnetic nanoparticles, achieving the simultaneous detection of different types of HPV by the fingerprint r

英文关键词： single-particle Raman readout；controlled assembly；detection；imaging；