基于上转换发光超结构复合物的编码载体构建与多元生物检测研究

项目名称： 基于上转换发光超结构复合物的编码载体构建与多元生物检测研究

项目编号： No.21305126

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 数理科学和化学

项目作者： 张庆彬

作者单位： 浙江工业大学

项目金额： 25万元

中文摘要： 稀土掺杂上转换发光纳米晶由于其近红外激发至可见光的发光特性在生物学检测中具有极低的生物体背景荧光、高的检测分辨率与灵敏度。本项目提出以特定上转换发光性质的NaYF4:Yb/Ln纳米晶为发光基元，构建上转换发光超结构复合光谱编码载体。采用简易的微乳液组装技术，利用纳米晶表面配体间的疏水相互作用和范德华力形成稳定的超结构复合体，通过精确控制体系内纳米晶的种类与组份调谐微球不同的特征发光谱带而获得大数量具有高确定性光谱地址的编码载体。并选用双亲性聚合物表面包覆修饰提高超结构编码粒子稳定性的同时使其具有水溶性及赋予适于生物结合的功能基团，利用共价偶联特异性分子构建检测探针用于高通量、高灵敏度和快速筛选肿瘤标志物的上转换均相悬浮检测，此工作可预期在药物筛选、医学临床诊断领域具有重要的科学价值。

中文关键词： 上转换发光；配体交换；表面包覆；生物标记；复合微球

英文摘要： Up-converting rare-earth nanoparticles have shown great potential as a new class of fluorophores for biological detection applications because of low background fluorescence under near infrared excitation. The project coding carriers construction based on up-conversion luminescence nanoparticles, the carriers using for multiple biological detection. The mass fabrication of up-conversion luminescence-encoded complex were achieved by employing a facile, robust, and low-cost self-assembly in microemulsion droplets,which offered a convenient, straightforward, and reproducible technique. The barcode information can be predicted and designed by controlling lanthanide composition, dopant concentration, and mass content inside complex. Furthermore, the complex were encapsulated with an amphiphilic polymer , which not only enhances the stability of the up-conversion luminescence-encoded complex but also provides the biocompatibility for immobilization of biomolecules. In addition, their surface bioactivity was investigated by using protein immunoassay, which indicates the potential application of the up-conversion luminescence-encoded complex in biodetection and biomedicine.

英文关键词： upconversion luminescence；ligand exchange；surface coating；biological labeling；composite microspheres