酿酒酵母发酵菊芋源己糖生产乙醇过程中果糖的代谢调控机制研究

项目名称： 酿酒酵母发酵菊芋源己糖生产乙醇过程中果糖的代谢调控机制研究

项目编号： No.21306221

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 化学工业

项目作者： 李莉莉

作者单位： 中国科学院烟台海岸带研究所

项目金额： 25万元

中文摘要： 酿酒酵母生产乙醇的原料很多，但是菊芋因其抗逆高产及高菊粉含量而备受关注。菊粉是果糖通过(β-2,1)糖苷键联接而成并以葡萄糖单元终止的果聚糖混合物。在菊粉水解液（果糖/葡萄糖比例约为4:1）乙醇发酵过程中，果糖的利用问题是影响菊芋生物转化的瓶颈之一。目前对己糖转运方面的研究较多，且主要集中在对己糖转运蛋白及其编码基因等方面的研究，对果糖的代谢调控缺少专一性的研究。在果糖占很大比例的菊芋源己糖这种特殊生物质的利用中，果糖的代谢调控机制，包括葡萄糖对果糖利用的调控机制都尚未知晓。本课题拟通过U-13C标记代谢通量及全基因组分析,从果糖转运、磷酸化及下游代谢三个模块进行分析，找到果糖代谢的限制性通道，确定限制果糖代谢的关键通路及基因，为菊芋及富含菊粉生物质的利用提供方向。

中文关键词： 菊芋；酿酒酵母；全基因组；代谢分析；果糖

英文摘要： Jerusalem artichoke (Helianthus tuberosus L.) does not compete with grain crops for arable land and is superior to the other crops in terms of its output of biomass production and inulin content. Inulin consists of linear chains of β-2,1-linked D-furanose molecules terminated by a glucose residue through a sucrose-type linkage at the reducing end. When JA is to be used for ethanol fermentation by Saccharomyces cerevisiae it is first converted to fermentable sugars by enzymes or acid. In inulin-derived sugars, the ratio of the fructose to glucose is about 4:1. The bottleneck of bio-conversion of Jerusalem artichoke is the utilization of fructose. To date, most researches focused on the hexose transport, especially the transporter and its related genes. However, there is little information about the regulation mechanism of fructose metabolites (distinguished from glucose) in ethanol fermentation with Jerusalem artichoke-derived hexose. Insights into regulation mechanism of fructose metabolites, including the regulation roles of glucose, were obtained by analyzing the regulation mechanism of fructose transport, phosphorylation and downstream metabolic pathway. Metabolic flux analysis with 13C labeling was combined with the expression profiles to investigate the key genes and pathways in the fructose metabolites to

英文关键词： Jerusalem artichoke；Saccharomyces cerevisiae；whole genome analysis；metabolic analysis；fructose