牛虻唾液腺中一种新型免疫抑制肽结构，功能及作用机制研究

项目名称： 牛虻唾液腺中一种新型免疫抑制肽结构，功能及作用机制研究

项目编号： No.31201717

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 生物科学

项目作者： 严秀文

作者单位： 南京农业大学

项目金额： 23万元

中文摘要： 牛虻（虻虫）是我国重要的中药材，牛虻唾液腺在长期的进化过程中形成免疫调节因子以逃避宿主免疫反应，从而保证成功地从宿主获得血液。我们从拟黑腹牛虻的唾液腺中分离纯化得到一种新型的含有30个氨基酸残基的免疫抑制肽immunoregulin HA。前期工作表明immunoregulin HA可能是通过上调IL-10的产生来抑制炎症因子IFN-γ和MCP-1的分泌。本课题首先将研究免疫抑制肽immunoregulin HA可能的结构及关键氨基酸位点。其次从分子水平、细胞水平深入研究抑制肽immunoregulin HA对几类免疫细胞的增殖、凋亡、分化的调控及可能的作用机制。最后利用动物模型评估免疫抑制肽immunoregulin HA的体内活性，为改造和设计新型免疫抑制剂分子打下基础。

中文关键词： 免疫抑制肽；信号通路；炎症模型；作用机制；

英文摘要： Horsefly is widely used in traditional Chinese medicine. As a blood-sucking arthropods, horsefly has developed many immunoregulator compounds in salivary gland in order to withstand the host immune response and suck blood successfully from the host after the lone-term evolution period. We purified a new immunosuppressive peptide named immunoregulin HA from the salivary gland extract of Hybomitra atriperoides. It is composed of 30 amino acid residues. Immunoregulin HA possibly upregulates the IL-10 production to inhibit inflammatory cytokines IFN-γ and MCP-1 secretion. In our next work, we will firstly focus on the stucture and the key amino acide sites of the immunosupressive HA. Secondly, We will try to find out its regulation on different immune cells in cell proliferation, apoptosis, differentiation and the possible mechanisms at both molecular level and cell level. Finally, we will use animal models to evaluate immunosuppressive peptide immunoregulin HA's activity in vivo, thus to make it possible to reform and design newly immunosuppressive molecules in future.

英文关键词： immunoinhibitory peptide；signal pathway；inflammation modle；mechanism；