广西苗族、侗族儿童乙肝疫苗免疫应答与TLR2、TLR4基因多态性关联的研究

项目名称： 广西苗族、侗族儿童乙肝疫苗免疫应答与TLR2、TLR4基因多态性关联的研究

项目编号： No.81360443

项目类型： 地区科学基金项目

立项/批准年度： 2014

项目学科： 医药、卫生

项目作者： 李海

作者单位： 广西壮族自治区疾病预防控制中心

项目金额： 50万元

中文摘要： .接种乙肝疫苗是预防HBV感染的最有效措施。2006年我国5 岁以下儿童HBsAg 感染率已降至低于1％，但2011年广西同龄儿童HBsAg感染率仍高达3.62%，乙肝疫苗无应答率为34.20%，远高于其它省的15～20%，尤其是苗族和侗族同龄儿童更高，分别为63.60%和45.80%，其原因值得深入研究。最新研究表明Toll 样受体2(TLR2) 和4(TLR4)的基因多态性(SNPs)与疫苗免疫应答有关，但TLR2和TLR4基因SNPs与苗族、侗族儿童乙肝疫苗免疫应答的关联尚未明确。.本研究采用1:1病例对照研究方法，以完成全程接种乙肝疫苗后的广西苗族和侗族儿童作为研究对象，分别选择低/无应答者、正常应答者作为病例和对照，采用PCR和SNaPshot技术做基因分型，从群体水平探讨TLR2、TLR4基因SNPs与乙肝疫苗免疫应答的关联，为了解乙肝疫苗免疫低/无应答的遗传学机制提供科学依据。

中文关键词： 乙肝疫苗;Toll样受体;单核苷酸多态性;免疫应答;关联研究

英文摘要： Hepatitis B vaccination is the most efective messure for prevention of Hepatitis B Virus(HBV) infection. Although the prevalance of surface antigen of the hepatitis B virus(HBsAg) was lower than 1% in children under 5 years old in China，a study performed in 2011 showed that the prevalance of HBsAg was 3.62% in children under 5 years old in Guangxi, and 34.2% children under 5 years old had no response to hepatitis B vaccination, which was higher than that of other province in China(15-20%).The non response rate to hepatitis B vaccination in Miao and Dong children was extrmely high(63.6% and 45.8%, respectively). This phenomenon should be further studied.New study confirmed that Toll-like receptors 2(TLR2) and Toll-like receptors 4(TLR4) single-nucleotide polymorphism(SNPs) are associated with immune response of vaccine, however, the association between SNPs of TLR2 and TLR4 and hepatitis B vaccination in Miao and Dong children were not demonstrated. .In this study, we perform a hospital base 1:1 case control study in Miao and Dong children in Guangxi after Hepatitis B vaccination.The children who have low or no response to hepatitis B vaccine are enroled as cases, while those who have normal response are enroled as controls. The SNPs of TLR2 and TL4 are genotyped by SNaPshot.We explore the association between SNPs of TLR2 and TLR4 gene and immune response to hepatitis B vaccine in a large population, which would provide scientific evidence for the genetics mechanism of low or no immune response to hepatitis B vaccine.

英文关键词： Hepatitis B vaccine;Toll like receptor;Single-nucleotide polymorphism(SNPs);immune response;Association study