TLR2和TLR4配体激活对新型结核疫苗AdAg85a免疫应答机制影响的研究

项目名称： TLR2和TLR4配体激活对新型结核疫苗AdAg85a免疫应答机制影响的研究

项目编号： No.81202375

项目类型： 青年科学基金项目

立项/批准年度： 2013

项目学科： 医学免疫学、法医学

项目作者： 陈雪融

作者单位： 四川大学

项目金额： 23万元

中文摘要： 新型结核疫苗AdAg85a已进入临床，但在宿主肺部细菌感染病理情况下的免疫保护作用及相关机理仍不清楚。本课题拟探讨TLR2或TLR4的激活对Ad5Ag85a吸入免疫小鼠气道腔内抗原特异性T淋巴细胞数目和功能之影响以及影响背后的机制，同时了解此种激活对疫苗针对结核杆菌保护作用的影响。方法学上拟以TLR2配体PGN或TLR4配体LPS暴露于AdAg85a吸入免疫BALB/c小鼠气道，或体外刺激AdAg85a免疫小鼠的气道腔内细胞，在不同时间点用四聚体技术检测气道腔抗原特异性CD8 T细胞数目，用细胞内细胞因子染色技术探测产生γ干扰素的CD4及CD8 T细胞数目，用ELISA法探讨气道腔内各细胞因子及化学素释放情况，同时通过结核菌培养了解结核杆菌攻击后免疫小鼠的肺脾菌落变化，最后以CFSE示踪、FTY720淋巴细胞供应阻断及TLR2或TLR4流式抗体染色等技术探索气道内T淋巴细胞变化之机理。

中文关键词： 内毒素；结核；疫苗；气道腔内T淋巴细胞；

英文摘要： We try to invstigate the effect of the activation of TLR2 or TLR4 on the number and function of antigen specific T cells in airway lumen in mice immunized with AdAg85a intranasally and the mechanism behind it. Furthermore, we are interested in investigating the impact of activation of such receptors on the role of the vaccine against tuberculosis. According to our plan, BALB/c mice will be immunized with AdAg85a intranasally. Peptidoglycan(TLR2 ligand) or lipopolysaccharide (TLR4 ligand) will stimulate the bronchoavelor cells of the vaccinated mice in vivo and in vitro. Antigen specific T cells in airway lumen will be detected by tetramer technology at different time points. IFN-γ producing CD4 and CD8 T cells will be detected by intracellular cytokine staining. The levels of cytokines and chemokines in bronchoaveolar fluid will be measured by ELISA. Colonies of mycobacteria in the lungs and spleens in vaccinated mice after challenge with H37Rv or H37Ra will be counted. The mechanism of change of antigen specific T cells in airway lumen will be explored by cell labeling with CFSE，administration of FTY720 and staining with TLR2 and TLR4 antibody for flow cytometry.

英文关键词： tuberculosis；airway luminal T cells；vaccine；TLR2；TLR4