光与激素共同调控植物发育的信号转导机制研究

项目名称： 光与激素共同调控植物发育的信号转导机制研究

项目编号： No.31330048

项目类型： 重点项目

立项/批准年度： 2014

项目学科： 生物科学

项目作者： 邓兴旺

作者单位： 北京大学

项目金额： 304万元

中文摘要： 光是调控植物生长发育十分重要的环境因子，植物激素是植物体内调控植物生长发育的内源小分子。已有研究表明，光与植物激素共同调控植物幼苗的光形态建成，其信号机制却不完全清楚。本项目研究分为三个部分：1）确认EIN3/EIL1这一类新发现的光受体直接相互作用转录因子，鉴定其在光形态建成中的作用，并分析它们与已知的PIFs家族转录因子在调控光形态建成中的关系，完善对植物光形态建成中转录因子调控的认识。2）通过对植物激素乙烯下游调控基因的分析，鉴定乙烯调控叶绿素合成、叶绿体发育和子叶扩展的功能基因，阐述乙烯调控光形态建成的信号机制。3）研究乙烯、赤霉素和油菜素内酯这三大植物激素怎样共同调控植物光形态建成，确定这些植物激素在调控光形态建成中的核心组分以及它们之间的关系，构建植物激素调控光形态建成的信号网络。这些研究将大大加深我们对植物如何在生长发育中整合外源光信号与内源植物激素调控的信号机制的认识。

中文关键词： 光形态建成;信号转导;光信号;植物激素;转录因子

英文摘要： Light is one of the most important environmental factors that regulate plant growth and development. Phytohormones, endogenous plant-made small molecules such as ethylene, regulate a wide range of processes throughout the lifetime of plants. Previous studies showed that plants integrate external light signal and internal plant hormones to regulate young seedlings’ photomorphogenesis. However, the underling mechanism is largely unknown. This study includes: 1) The direct interaction of phytochromes and EIN3/EIL1 proteins will be further confirmed and how light and phytochromes regulate EIN3/EIL1’s function will be investigated. We will construct multiple mutations of EIN3/EIL1 and PIFs to test how the EIN3/EIL1 cooperates with PIFs in regulating plant photomorphogenesis. 2) High-throughput mRNA sequence method will be used to analyze ethylene regulated downstream genes. Genes regulating chlorophyll synthesis, chloroplast development and cotyledon expansion processes will be identified, to elucidate the molecular mechanism of how ethylene regulates plant photomorphogenesis. 3) How the three known phytohormones (ethylene, gibberellin and brassinosteroid) regulate photomorphogenesis together will be investigated. We will try to find out the common components of plant hormones and light signal pathways in this regulation process. Then we will examine the relationship of these components and build up the molecular network for phytohormone-regulated photomorphogenesis. These studies will dramatically improve our understanding about how plants integrate environmental factors and endogenous phytohormones to elaborately regulate plant development.

英文关键词： Photomorphogenesis;Signal Transduction;Light signal;Phytohormones;Transcription factors